Angiogenesis in the ischemic rat lung.

Research output: Contribution to journalArticle

Abstract

The adult lung is perfused by both the systemic bronchial artery and the entire venous return flowing through the pulmonary arteries. In most lung pathologies, it is the smaller systemic vasculature that responds to a need for enhanced lung perfusion and shows robust neovascularization. Pulmonary vascular ischemia induced by pulmonary artery obstruction has been shown to result in rapid systemic arterial angiogenesis in man as well as in several animal models. Although the histologic assessment of the time course of bronchial artery proliferation in rats was carefully described by Weibel , mechanisms responsible for this organized growth of new vessels are not clear. We provide surgical details of inducing left pulmonary artery ischemia in the rat that leads to bronchial neovascularization. Quantification of the extent of angiogenesis presents an additional challenge due to the presence of the two vascular beds within the lung. Methods to determine functional angiogenesis based on labeled microsphere injections are provided.

Original languageEnglish (US)
JournalJournal of Visualized Experiments
Issue number72
StatePublished - 2013
Externally publishedYes

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Rats
Lung
Bronchial Arteries
Pulmonary Artery
Pathology
Microspheres
Animals
Blood Vessels
Ischemia
Animal Models
Perfusion
Injections
Growth

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Angiogenesis in the ischemic rat lung. / Jenkins, John; Wagner, Elizabeth Marie.

In: Journal of Visualized Experiments, No. 72, 2013.

Research output: Contribution to journalArticle

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