TY - JOUR
T1 - Angiogenesis in developing rat brain
T2 - An in vivo and in vitro study
AU - Robertson, Patricia L.
AU - Du Bois, Monica
AU - Bowman, Phillip D.
AU - Goldstein, Gary W.
N1 - Funding Information:
P.L.R. and M.Dw. eres upportebdy NINCDS Na tional ResearchS erviceA ward 5T32NS07222T.h ~ work was supportebdy Grant ES 02380f romthe Na tionalI nstituteo f Health.
PY - 1985/12
Y1 - 1985/12
N2 - Brain capillary proliferation in postnatal rats was measured in vivo by [3H]thymidine autoradiography. Maximal capillary proliferation occurred between 5 and 9 postnatal days, and was 40 times greater than in the adult. To test the hypothesis that soluble angiogenesis factors play a role in this developmental vascularization of brain, we prepared extracts from the brains of 6-day-old rats at the peak of proliferative activity, and from adults when it was lowest. We assayed them using an in vitro growth system measuring [3H]thymidine incorporation into cultured brain capillary endothelial cells. Extracts prepared from either 6-day or adult rats and containing 150 μg/ml protein caused more than a 4-fold stimulation of the endothelial cells, increasing to 8-fold at a concentration of 1500 μg/ml. The presence of growth-promoting activity in brain extracts from both adult and immature rats suggests that soluble angiogenesis factors may be present in the brain throughout life, but are unavailable for stimulation of in vivo capillary growth unless released or activated by an appropriate stimulus.
AB - Brain capillary proliferation in postnatal rats was measured in vivo by [3H]thymidine autoradiography. Maximal capillary proliferation occurred between 5 and 9 postnatal days, and was 40 times greater than in the adult. To test the hypothesis that soluble angiogenesis factors play a role in this developmental vascularization of brain, we prepared extracts from the brains of 6-day-old rats at the peak of proliferative activity, and from adults when it was lowest. We assayed them using an in vitro growth system measuring [3H]thymidine incorporation into cultured brain capillary endothelial cells. Extracts prepared from either 6-day or adult rats and containing 150 μg/ml protein caused more than a 4-fold stimulation of the endothelial cells, increasing to 8-fold at a concentration of 1500 μg/ml. The presence of growth-promoting activity in brain extracts from both adult and immature rats suggests that soluble angiogenesis factors may be present in the brain throughout life, but are unavailable for stimulation of in vivo capillary growth unless released or activated by an appropriate stimulus.
KW - [H]thymidine autoradiography
KW - angiogenesis
KW - cultured endothelial cells
KW - endothelial cell growth factor
KW - rat brain development
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U2 - 10.1016/0165-3806(85)90044-6
DO - 10.1016/0165-3806(85)90044-6
M3 - Article
C2 - 4084777
AN - SCOPUS:0022296245
SN - 0165-3806
VL - 23
SP - 219
EP - 223
JO - Developmental Brain Research
JF - Developmental Brain Research
IS - 2
ER -