Angiogenesis-associated crosstalk between collagens, CXC chemokines, and thrombospondin domain-containing proteins

Corban G. Rivera, Joel S. Bader, Aleksander S. Popel

Research output: Contribution to journalArticle

Abstract

Excessive vascularization is a hallmark of many diseases including cancer, rheumatoid arthritis, diabetic nephropathy, pathologic obesity, age-related macular degeneration, and asthma. Compounds that inhibit angiogenesis represent potential therapeutics for many diseases. Karagiannis and Popel [Proc. Natl. Acad. Sci. USA 105(37):13775-13780, 2008] used a bioinformatics approach to identify more than 100 peptides with sequence homology to known angiogenesis inhibitors. The peptides could be grouped into families by the conserved domain of the proteins they were derived from. The families included type IV collagen fibrils, CXC chemokine ligands, and type I thrombospondin domain-containing proteins. The relationships between these families have received relatively little attention. To investigate these relationships, we approached the problem by placing the families of proteins in the context of the human interactome including >120,000 physical interactions among proteins, genes, and transcripts. We built on a graph theoretic approach to identify proteins that may represent conduits of crosstalk between protein families. We validated these findings by statistical analysis and analysis of a time series gene expression data set taken during angiogenesis. We identified six proteins at the center of the angiogenesis-associated network including three syndecans, MMP9, CD44, and versican. These findings shed light on the complex signaling networks that govern angiogenesis phenomena.

Original languageEnglish (US)
Pages (from-to)2213-2222
Number of pages10
JournalAnnals of biomedical engineering
Volume39
Issue number8
DOIs
StatePublished - Aug 1 2011

    Fingerprint

Keywords

  • Angiogenesis
  • CXC chemokine
  • Crosstalk
  • Interactome
  • Syndecan
  • Thrombospondin-1
  • Type IV collagen

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this