Anesthetic propofol normalized the increased release of glutamate and γ-amino butyric acid in hippocampus after paradoxical sleep deprivation in rats

Fang Xie, Xueyang Li, Mengmeng Bao, Rong Shi, Yun Yue, Yun Guan, Yun Wang

Research output: Contribution to journalArticle

Abstract

Objective: Multiple lines of evidence suggest that general anesthesia helps the recovery from sleep deprivation.However, little is known about the underlying neurochemical mechanisms. In the current study, we investigated theeffect of anesthetic propofol on the release of glutamate (Glu) and γ-amino butyric acid (GABA) in the hippocampalCA1 region of rat with 24 h-paradoxical sleep deprivation (PSD). Methods: A guide cannula for microdialysis was inserted into the CA1 region of hippocampus in rats. At six daysafter cannula implantation, rats received 24 h-PSD by using the platform–water tank method. The rats were thensubjected to natural sleep or propofol anesthesia (100 mg/kg, i.p.), respectively, after 24-h PSD. Microdialysissamples from hippocampus were collected before and at the end of PSD, and also at 1, 3, 6, and 8 h post-PSD.The concentrations of Glu and GABA in collected samples were determined by using high performance liquidchromatography. Results: The current study showed that 24 h-PSD significantly increased the release of Glu and GABA in the hippocampus in rats. In both natural sleep and propofol anesthesia groups, the upregulated Glu and GABA levelsafter PSD gradually decreased and returned to the baseline level by 8 h post-PSD. Conclusion: Our data indicate that propofol anesthesia promotes the restoration of disturbed excitatory andinhibitory neurotransmitter release in the hippocampus after PSD, similar to the beneficial effects of natural sleep. This finding suggests that propofol anesthesia may be a viable pharmacotherapeutic strategy for the treatmentof certain sleep disorders that share similar mechanisms with PSD.

Original languageEnglish (US)
Pages (from-to)1102-1107
Number of pages6
JournalNeurological research
Volume37
Issue number12
DOIs
StatePublished - Dec 2015

Keywords

  • Anesthesia
  • Hippocampus
  • Neurochemistry
  • Sleep deprivation

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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