Anesthetic agents isoflurane and propofol decrease maximal Ca21-activated force and thus contractility in the failing myocardium

Tao Meng, Xianfeng Ren, Xinzhong Chen, Jingui Yu, Jacopo Agrimi, Nazareno Paolocci, Wei Dong Gao

Research output: Contribution to journalArticlepeer-review

Abstract

In the normal heart, frequently used anesthetics such as isoflurane and propofol can reduce inotropy. However, the impact of these agents on the failing myocardium is unclear. Here, we examined whether and how isoflurane and propofol influence cardiac contractility in intact cardiac muscles from rats treated with monocrotaline to induce heart failure. We measured force and intracellular Ca21 ([Ca21]i) in trabeculae from the right ventricles of the rats in the absence or presence of propofol or isoflurane. At low to moderate concentrations, both propofol and isoflurane dose-dependently depressed cardiac force generation in failing trabeculae without altering [Ca21]i. At high doses, propofol (but not isoflurane) also decreased amplitude of [Ca21]i transients. During steady-state activation, both propofol and isoflurane impaired maximal Ca21activated force (Fmax) while increasing the amount of [Ca21]i required for 50% of maximal activation (Ca50). These events occurred without apparent change in the Hill coefficient, suggesting no impairment of cooperativity. Exposing these same muscles to the anesthetics after fiber skinning resulted in a similar decrement in Fmax and rise in Ca50 but no change in the myofibrillar ATPase-Ca21 relationship. Thus, our study demonstrates that challenging the failing myocardium with commonly used anesthetic agents such as propofol and isoflurane leads to reduced force development as a result of lowered myofilament responsiveness to Ca21

Original languageEnglish (US)
Pages (from-to)615-623
Number of pages9
JournalJournal of Pharmacology and Experimental Therapeutics
Volume371
Issue number3
DOIs
StatePublished - 2019

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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