Androgen regulation of JM-27 is associated with the diseased prostate

Uzma S. Shah, Julie Arlotti, Rajiv Dhir, Sun Lu, Gregorio Pirozzi, Kulkarni Prakash, Robert H. Getzenberg

Research output: Contribution to journalArticle

Abstract

Despite intense research efforts, the etiology of prostatic hyperplasia associated with both benign prostatic hyperplasia (BPH) and prostate cancer remains poorly understood. Our previous studies using array technology identified JM-27 as a transcript that is dramatically up-regulated in the prostates of patients with symptomatic BPH and in normal, adjacent prostatic regions of patients with prostate cancer. In the present study, using an extended sample set, we show a correlation between the messenger RNA and protein expression of JM-27. To investigate the possible functions of this gene, its expression in the rat prostate was examined by immunoblot analysis using a polyclonal antibody specific to human JM-27. This antibody reacts with 2 rat polypeptides of 17 kd and 27 kd in size. Whereas the 27-kd form of the JM-27 protein found in human prostate is selectively expressed in the dorsolateral lobes of the rat prostate, the 17-kd form is expressed only in the ventral lobe. Expression of both forms of this protein appears to be androgen-regulated. There is a time-dependent decrease in expression of the protein products in the ventral and dorsolateral lobes of the rat prostate after castration. Administration of exogenous testosterone in castrated animals maintains protein expression in both lobes. Androgens are believed to play a central role in prostate growth and development, and therefore, it is tempting to speculate that JM-27, an androgen-regulated gene, may be involved in prostatic growth regulation. Further studies are underway to evaluate such a function for JM-27 in prostatic diseases.

Original languageEnglish (US)
Pages (from-to)618-624
Number of pages7
JournalJournal of Andrology
Volume25
Issue number4
StatePublished - Jul 2004
Externally publishedYes

Fingerprint

Androgens
Prostate
Prostatic Hyperplasia
Prostatic Neoplasms
Proteins
Prostatic Diseases
Antibodies
Castration
Growth and Development
Testosterone
Technology
Gene Expression
Messenger RNA
Peptides
Growth
Research
Genes

Keywords

  • Benign prostatic hyperplasia
  • Gene expression
  • Genes
  • Prostate cancer

ASJC Scopus subject areas

  • Endocrinology

Cite this

Shah, U. S., Arlotti, J., Dhir, R., Lu, S., Pirozzi, G., Prakash, K., & Getzenberg, R. H. (2004). Androgen regulation of JM-27 is associated with the diseased prostate. Journal of Andrology, 25(4), 618-624.

Androgen regulation of JM-27 is associated with the diseased prostate. / Shah, Uzma S.; Arlotti, Julie; Dhir, Rajiv; Lu, Sun; Pirozzi, Gregorio; Prakash, Kulkarni; Getzenberg, Robert H.

In: Journal of Andrology, Vol. 25, No. 4, 07.2004, p. 618-624.

Research output: Contribution to journalArticle

Shah, US, Arlotti, J, Dhir, R, Lu, S, Pirozzi, G, Prakash, K & Getzenberg, RH 2004, 'Androgen regulation of JM-27 is associated with the diseased prostate', Journal of Andrology, vol. 25, no. 4, pp. 618-624.
Shah US, Arlotti J, Dhir R, Lu S, Pirozzi G, Prakash K et al. Androgen regulation of JM-27 is associated with the diseased prostate. Journal of Andrology. 2004 Jul;25(4):618-624.
Shah, Uzma S. ; Arlotti, Julie ; Dhir, Rajiv ; Lu, Sun ; Pirozzi, Gregorio ; Prakash, Kulkarni ; Getzenberg, Robert H. / Androgen regulation of JM-27 is associated with the diseased prostate. In: Journal of Andrology. 2004 ; Vol. 25, No. 4. pp. 618-624.
@article{8c834b2b991d436b8ff5bd882c6bfbc6,
title = "Androgen regulation of JM-27 is associated with the diseased prostate",
abstract = "Despite intense research efforts, the etiology of prostatic hyperplasia associated with both benign prostatic hyperplasia (BPH) and prostate cancer remains poorly understood. Our previous studies using array technology identified JM-27 as a transcript that is dramatically up-regulated in the prostates of patients with symptomatic BPH and in normal, adjacent prostatic regions of patients with prostate cancer. In the present study, using an extended sample set, we show a correlation between the messenger RNA and protein expression of JM-27. To investigate the possible functions of this gene, its expression in the rat prostate was examined by immunoblot analysis using a polyclonal antibody specific to human JM-27. This antibody reacts with 2 rat polypeptides of 17 kd and 27 kd in size. Whereas the 27-kd form of the JM-27 protein found in human prostate is selectively expressed in the dorsolateral lobes of the rat prostate, the 17-kd form is expressed only in the ventral lobe. Expression of both forms of this protein appears to be androgen-regulated. There is a time-dependent decrease in expression of the protein products in the ventral and dorsolateral lobes of the rat prostate after castration. Administration of exogenous testosterone in castrated animals maintains protein expression in both lobes. Androgens are believed to play a central role in prostate growth and development, and therefore, it is tempting to speculate that JM-27, an androgen-regulated gene, may be involved in prostatic growth regulation. Further studies are underway to evaluate such a function for JM-27 in prostatic diseases.",
keywords = "Benign prostatic hyperplasia, Gene expression, Genes, Prostate cancer",
author = "Shah, {Uzma S.} and Julie Arlotti and Rajiv Dhir and Sun Lu and Gregorio Pirozzi and Kulkarni Prakash and Getzenberg, {Robert H.}",
year = "2004",
month = "7",
language = "English (US)",
volume = "25",
pages = "618--624",
journal = "Journal of Andrology",
issn = "0196-3635",
publisher = "American Society of Andrology Inc.",
number = "4",

}

TY - JOUR

T1 - Androgen regulation of JM-27 is associated with the diseased prostate

AU - Shah, Uzma S.

AU - Arlotti, Julie

AU - Dhir, Rajiv

AU - Lu, Sun

AU - Pirozzi, Gregorio

AU - Prakash, Kulkarni

AU - Getzenberg, Robert H.

PY - 2004/7

Y1 - 2004/7

N2 - Despite intense research efforts, the etiology of prostatic hyperplasia associated with both benign prostatic hyperplasia (BPH) and prostate cancer remains poorly understood. Our previous studies using array technology identified JM-27 as a transcript that is dramatically up-regulated in the prostates of patients with symptomatic BPH and in normal, adjacent prostatic regions of patients with prostate cancer. In the present study, using an extended sample set, we show a correlation between the messenger RNA and protein expression of JM-27. To investigate the possible functions of this gene, its expression in the rat prostate was examined by immunoblot analysis using a polyclonal antibody specific to human JM-27. This antibody reacts with 2 rat polypeptides of 17 kd and 27 kd in size. Whereas the 27-kd form of the JM-27 protein found in human prostate is selectively expressed in the dorsolateral lobes of the rat prostate, the 17-kd form is expressed only in the ventral lobe. Expression of both forms of this protein appears to be androgen-regulated. There is a time-dependent decrease in expression of the protein products in the ventral and dorsolateral lobes of the rat prostate after castration. Administration of exogenous testosterone in castrated animals maintains protein expression in both lobes. Androgens are believed to play a central role in prostate growth and development, and therefore, it is tempting to speculate that JM-27, an androgen-regulated gene, may be involved in prostatic growth regulation. Further studies are underway to evaluate such a function for JM-27 in prostatic diseases.

AB - Despite intense research efforts, the etiology of prostatic hyperplasia associated with both benign prostatic hyperplasia (BPH) and prostate cancer remains poorly understood. Our previous studies using array technology identified JM-27 as a transcript that is dramatically up-regulated in the prostates of patients with symptomatic BPH and in normal, adjacent prostatic regions of patients with prostate cancer. In the present study, using an extended sample set, we show a correlation between the messenger RNA and protein expression of JM-27. To investigate the possible functions of this gene, its expression in the rat prostate was examined by immunoblot analysis using a polyclonal antibody specific to human JM-27. This antibody reacts with 2 rat polypeptides of 17 kd and 27 kd in size. Whereas the 27-kd form of the JM-27 protein found in human prostate is selectively expressed in the dorsolateral lobes of the rat prostate, the 17-kd form is expressed only in the ventral lobe. Expression of both forms of this protein appears to be androgen-regulated. There is a time-dependent decrease in expression of the protein products in the ventral and dorsolateral lobes of the rat prostate after castration. Administration of exogenous testosterone in castrated animals maintains protein expression in both lobes. Androgens are believed to play a central role in prostate growth and development, and therefore, it is tempting to speculate that JM-27, an androgen-regulated gene, may be involved in prostatic growth regulation. Further studies are underway to evaluate such a function for JM-27 in prostatic diseases.

KW - Benign prostatic hyperplasia

KW - Gene expression

KW - Genes

KW - Prostate cancer

UR - http://www.scopus.com/inward/record.url?scp=3042814437&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042814437&partnerID=8YFLogxK

M3 - Article

C2 - 15223850

AN - SCOPUS:3042814437

VL - 25

SP - 618

EP - 624

JO - Journal of Andrology

JF - Journal of Andrology

SN - 0196-3635

IS - 4

ER -