Abstract
Despite intense research efforts, the etiology of prostatic hyperplasia associated with both benign prostatic hyperplasia (BPH) and prostate cancer remains poorly understood. Our previous studies using array technology identified JM-27 as a transcript that is dramatically up-regulated in the prostates of patients with symptomatic BPH and in normal, adjacent prostatic regions of patients with prostate cancer. In the present study, using an extended sample set, we show a correlation between the messenger RNA and protein expression of JM-27. To investigate the possible functions of this gene, its expression in the rat prostate was examined by immunoblot analysis using a polyclonal antibody specific to human JM-27. This antibody reacts with 2 rat polypeptides of 17 kd and 27 kd in size. Whereas the 27-kd form of the JM-27 protein found in human prostate is selectively expressed in the dorsolateral lobes of the rat prostate, the 17-kd form is expressed only in the ventral lobe. Expression of both forms of this protein appears to be androgen-regulated. There is a time-dependent decrease in expression of the protein products in the ventral and dorsolateral lobes of the rat prostate after castration. Administration of exogenous testosterone in castrated animals maintains protein expression in both lobes. Androgens are believed to play a central role in prostate growth and development, and therefore, it is tempting to speculate that JM-27, an androgen-regulated gene, may be involved in prostatic growth regulation. Further studies are underway to evaluate such a function for JM-27 in prostatic diseases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 618-624 |
| Number of pages | 7 |
| Journal | Journal of Andrology |
| Volume | 25 |
| Issue number | 4 |
| State | Published - Jul 2004 |
| Externally published | Yes |
Fingerprint
Keywords
- Benign prostatic hyperplasia
- Gene expression
- Genes
- Prostate cancer
ASJC Scopus subject areas
- Endocrinology
Cite this
Androgen regulation of JM-27 is associated with the diseased prostate. / Shah, Uzma S.; Arlotti, Julie; Dhir, Rajiv; Lu, Sun; Pirozzi, Gregorio; Prakash, Kulkarni; Getzenberg, Robert H.
In: Journal of Andrology, Vol. 25, No. 4, 07.2004, p. 618-624.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Androgen regulation of JM-27 is associated with the diseased prostate
AU - Shah, Uzma S.
AU - Arlotti, Julie
AU - Dhir, Rajiv
AU - Lu, Sun
AU - Pirozzi, Gregorio
AU - Prakash, Kulkarni
AU - Getzenberg, Robert H.
PY - 2004/7
Y1 - 2004/7
N2 - Despite intense research efforts, the etiology of prostatic hyperplasia associated with both benign prostatic hyperplasia (BPH) and prostate cancer remains poorly understood. Our previous studies using array technology identified JM-27 as a transcript that is dramatically up-regulated in the prostates of patients with symptomatic BPH and in normal, adjacent prostatic regions of patients with prostate cancer. In the present study, using an extended sample set, we show a correlation between the messenger RNA and protein expression of JM-27. To investigate the possible functions of this gene, its expression in the rat prostate was examined by immunoblot analysis using a polyclonal antibody specific to human JM-27. This antibody reacts with 2 rat polypeptides of 17 kd and 27 kd in size. Whereas the 27-kd form of the JM-27 protein found in human prostate is selectively expressed in the dorsolateral lobes of the rat prostate, the 17-kd form is expressed only in the ventral lobe. Expression of both forms of this protein appears to be androgen-regulated. There is a time-dependent decrease in expression of the protein products in the ventral and dorsolateral lobes of the rat prostate after castration. Administration of exogenous testosterone in castrated animals maintains protein expression in both lobes. Androgens are believed to play a central role in prostate growth and development, and therefore, it is tempting to speculate that JM-27, an androgen-regulated gene, may be involved in prostatic growth regulation. Further studies are underway to evaluate such a function for JM-27 in prostatic diseases.
AB - Despite intense research efforts, the etiology of prostatic hyperplasia associated with both benign prostatic hyperplasia (BPH) and prostate cancer remains poorly understood. Our previous studies using array technology identified JM-27 as a transcript that is dramatically up-regulated in the prostates of patients with symptomatic BPH and in normal, adjacent prostatic regions of patients with prostate cancer. In the present study, using an extended sample set, we show a correlation between the messenger RNA and protein expression of JM-27. To investigate the possible functions of this gene, its expression in the rat prostate was examined by immunoblot analysis using a polyclonal antibody specific to human JM-27. This antibody reacts with 2 rat polypeptides of 17 kd and 27 kd in size. Whereas the 27-kd form of the JM-27 protein found in human prostate is selectively expressed in the dorsolateral lobes of the rat prostate, the 17-kd form is expressed only in the ventral lobe. Expression of both forms of this protein appears to be androgen-regulated. There is a time-dependent decrease in expression of the protein products in the ventral and dorsolateral lobes of the rat prostate after castration. Administration of exogenous testosterone in castrated animals maintains protein expression in both lobes. Androgens are believed to play a central role in prostate growth and development, and therefore, it is tempting to speculate that JM-27, an androgen-regulated gene, may be involved in prostatic growth regulation. Further studies are underway to evaluate such a function for JM-27 in prostatic diseases.
KW - Benign prostatic hyperplasia
KW - Gene expression
KW - Genes
KW - Prostate cancer
UR - http://www.scopus.com/inward/record.url?scp=3042814437&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042814437&partnerID=8YFLogxK
M3 - Article
C2 - 15223850
AN - SCOPUS:3042814437
VL - 25
SP - 618
EP - 624
JO - Journal of Andrology
JF - Journal of Andrology
SN - 0196-3635
IS - 4
ER -