Androgen glucuronides. I. Direct formation in rat accessory sex organs

Leland W K Chung, Donald S. Coffey

Research output: Contribution to journalArticle

Abstract

A simple one-step procedure is described on the isolation of androgen glucuronides from various rat tissues. This procedure uses polyacrylamide gel electrophoresis, and permits a quantitative isolation of a single band containing the total androgen glucuronides without the contamination of free androgens and androgen sulfates. This procedure was used to determine the ability of various tissues of the rat to form androgen glucuronides directly when they were incubated with 1,2-[3H]-testosterone (0.1 μM) in vitro. Of eleven organs studied, only the accessory sex organs (ventral prostate, seminal vesicle, and coagulating gland), liver, and kidney were capable of forming androgen glucuronides. At the end of a one-hour incubation period, approximately 1% of the total radiolabeled steroids in the prostatic tissue minces were in the form of glucuronide conjugates. The predominant androgen glucuronide formed in the accessory sex organs was 5α-androstane-3α,17β-diol 17β-d-glucuronide. This is in contrast to the rat liver and kidney in which testosterone glucuronide was the predominant conjugate. A similar amount of labeled glucuronide conjugates was formed from either [3H]-testosterone, [3H]-dihydrotestosterone or [3H]-androstenedione, whereas negligible amount of steroid conjugates was formed from [3H]-cortisol. The formation of androgen glucuronides requires metabolically active tissues; furthermore, the conjugation process was inhibited by the antiandrogen, cyproterone acetate, or by metabolic inhibitors, such as oligomycin or N-ethylmaleimide.

Original languageEnglish (US)
Pages (from-to)223-243
Number of pages21
JournalSteroids
Volume30
Issue number2
DOIs
StatePublished - 1977

Fingerprint

Genitalia
Glucuronides
Accessories
Androgens
Rats
Tissue
Liver
Androstane-3,17-diol
Testosterone
Steroids
Cyproterone Acetate
Kidney
Oligomycins
Androgen Antagonists
Ethylmaleimide
Seminal Vesicles
Androstenedione
Dihydrotestosterone
Electrophoresis
Sulfates

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Molecular Biology

Cite this

Androgen glucuronides. I. Direct formation in rat accessory sex organs. / Chung, Leland W K; Coffey, Donald S.

In: Steroids, Vol. 30, No. 2, 1977, p. 223-243.

Research output: Contribution to journalArticle

Chung, Leland W K ; Coffey, Donald S. / Androgen glucuronides. I. Direct formation in rat accessory sex organs. In: Steroids. 1977 ; Vol. 30, No. 2. pp. 223-243.
@article{277e0dad04d541d3af0b8d1623c324a1,
title = "Androgen glucuronides. I. Direct formation in rat accessory sex organs",
abstract = "A simple one-step procedure is described on the isolation of androgen glucuronides from various rat tissues. This procedure uses polyacrylamide gel electrophoresis, and permits a quantitative isolation of a single band containing the total androgen glucuronides without the contamination of free androgens and androgen sulfates. This procedure was used to determine the ability of various tissues of the rat to form androgen glucuronides directly when they were incubated with 1,2-[3H]-testosterone (0.1 μM) in vitro. Of eleven organs studied, only the accessory sex organs (ventral prostate, seminal vesicle, and coagulating gland), liver, and kidney were capable of forming androgen glucuronides. At the end of a one-hour incubation period, approximately 1{\%} of the total radiolabeled steroids in the prostatic tissue minces were in the form of glucuronide conjugates. The predominant androgen glucuronide formed in the accessory sex organs was 5α-androstane-3α,17β-diol 17β-d-glucuronide. This is in contrast to the rat liver and kidney in which testosterone glucuronide was the predominant conjugate. A similar amount of labeled glucuronide conjugates was formed from either [3H]-testosterone, [3H]-dihydrotestosterone or [3H]-androstenedione, whereas negligible amount of steroid conjugates was formed from [3H]-cortisol. The formation of androgen glucuronides requires metabolically active tissues; furthermore, the conjugation process was inhibited by the antiandrogen, cyproterone acetate, or by metabolic inhibitors, such as oligomycin or N-ethylmaleimide.",
author = "Chung, {Leland W K} and Coffey, {Donald S.}",
year = "1977",
doi = "10.1016/0039-128X(77)90084-8",
language = "English (US)",
volume = "30",
pages = "223--243",
journal = "Steroids",
issn = "0039-128X",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Androgen glucuronides. I. Direct formation in rat accessory sex organs

AU - Chung, Leland W K

AU - Coffey, Donald S.

PY - 1977

Y1 - 1977

N2 - A simple one-step procedure is described on the isolation of androgen glucuronides from various rat tissues. This procedure uses polyacrylamide gel electrophoresis, and permits a quantitative isolation of a single band containing the total androgen glucuronides without the contamination of free androgens and androgen sulfates. This procedure was used to determine the ability of various tissues of the rat to form androgen glucuronides directly when they were incubated with 1,2-[3H]-testosterone (0.1 μM) in vitro. Of eleven organs studied, only the accessory sex organs (ventral prostate, seminal vesicle, and coagulating gland), liver, and kidney were capable of forming androgen glucuronides. At the end of a one-hour incubation period, approximately 1% of the total radiolabeled steroids in the prostatic tissue minces were in the form of glucuronide conjugates. The predominant androgen glucuronide formed in the accessory sex organs was 5α-androstane-3α,17β-diol 17β-d-glucuronide. This is in contrast to the rat liver and kidney in which testosterone glucuronide was the predominant conjugate. A similar amount of labeled glucuronide conjugates was formed from either [3H]-testosterone, [3H]-dihydrotestosterone or [3H]-androstenedione, whereas negligible amount of steroid conjugates was formed from [3H]-cortisol. The formation of androgen glucuronides requires metabolically active tissues; furthermore, the conjugation process was inhibited by the antiandrogen, cyproterone acetate, or by metabolic inhibitors, such as oligomycin or N-ethylmaleimide.

AB - A simple one-step procedure is described on the isolation of androgen glucuronides from various rat tissues. This procedure uses polyacrylamide gel electrophoresis, and permits a quantitative isolation of a single band containing the total androgen glucuronides without the contamination of free androgens and androgen sulfates. This procedure was used to determine the ability of various tissues of the rat to form androgen glucuronides directly when they were incubated with 1,2-[3H]-testosterone (0.1 μM) in vitro. Of eleven organs studied, only the accessory sex organs (ventral prostate, seminal vesicle, and coagulating gland), liver, and kidney were capable of forming androgen glucuronides. At the end of a one-hour incubation period, approximately 1% of the total radiolabeled steroids in the prostatic tissue minces were in the form of glucuronide conjugates. The predominant androgen glucuronide formed in the accessory sex organs was 5α-androstane-3α,17β-diol 17β-d-glucuronide. This is in contrast to the rat liver and kidney in which testosterone glucuronide was the predominant conjugate. A similar amount of labeled glucuronide conjugates was formed from either [3H]-testosterone, [3H]-dihydrotestosterone or [3H]-androstenedione, whereas negligible amount of steroid conjugates was formed from [3H]-cortisol. The formation of androgen glucuronides requires metabolically active tissues; furthermore, the conjugation process was inhibited by the antiandrogen, cyproterone acetate, or by metabolic inhibitors, such as oligomycin or N-ethylmaleimide.

UR - http://www.scopus.com/inward/record.url?scp=0017708836&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017708836&partnerID=8YFLogxK

U2 - 10.1016/0039-128X(77)90084-8

DO - 10.1016/0039-128X(77)90084-8

M3 - Article

VL - 30

SP - 223

EP - 243

JO - Steroids

JF - Steroids

SN - 0039-128X

IS - 2

ER -