3H-labeled androgen glucuronide can be formed directly in tissue minces from normal human prostate and benign hyperplastic prostate when incubated with 1,2-3H-testosterone (0.2 μM). During a 1-hr incubation period, total androgen glucuronides formed directly in the tissue accounted for approximately 1.5 per cent of the total 3H-labeled androgens accumulated in the tissue. Subsequent chemical characterization of the androgen glucuronides revealed that 3H-testosterone glucuronide was the major glucuronide formed in the normal prostate, whereas 3H-dihydrotestosterone glucuronide was the major glucuronide conjugate formed in the hyperplastic prostate. The total amount of steroid glucuronides formed in the two types of tissue was similar. However, the ratio of total dihydrotestosterone glucuronide to total testosterone glucuronide found in benign hyperplastic prostate was 15, which was 19 fold higher than that observed in the normal prostate in which the ratio was 0.8. The antiandrogen, cyproterone acetate (12 μM), markedly inhibited the formation of androgen glucuronides by the benign hyperplastic prostate whereas only slightly decreased the accumulation of total 3H-labeled steroids in this tissue. Cyproterone acetate also significantly altered the pattern of androgen glucuronides formed directly in the tissue.
|Original language||English (US)|
|Number of pages||4|
|State||Published - Dec 1 1978|
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