Ancillary procedures necessary for translational research in experimental craniomaxillofacial surgery

Mohammed Al-Rakan, Jaimie T. Shores, Steve Bonawitz, Gabriel Santiago, Joani M. Christensen, Gerald Grant, Ryan J. Murphy, Ehsan Basafa, Mehran Armand, Pete Otovic, Sue Eller, Gerald Brandacher, Chad R. Gordon

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: Swine are often regarded as having analogous facial skeletons to humans and therefore serve as an ideal animal model for translational investigation. However, there is a dearth of literature describing the pertinent ancillary procedures required for craniomaxillofacial research. With this in mind, our objective was to evaluate all necessary procedures required for perioperative management and animal safety related to experimental craniomaxillofacial surgical procedures such as orthotopic, maxillofacial transplantation. Methods: Miniature swine (n = 9) were used to investigate perioperative airway management, methods for providing nutrition, and long-dwelling intravenous access. Flap perfusion using near-infrared laser angiography and facial nerve assessment with electromyoneurography were explored. Results: Bivona tracheostomy was deemed appropriate versus Shiley because soft, wire-reinforced tubing reduced the incidence of tracheal necrosis. Percutaneous endoscopic gastrostomy tube, as opposed to esophagostomy, provided a reliable route for postoperative feeding. Femoral venous access with dorsal tunneling proved to be an ideal option being far from pertinent neck vessels. Laser angiography was beneficial for real-time evaluation of graft perfusion. Facial electromyoneurography techniques for tracing capture were found most optimal using percutaneous leads near the oral commissure. Experience shows that ancillary procedures are critical, and malpositioning of devices may lead to irreversible sequelae with premature animal death. Conclusions: Face-jaw-teeth transplantation in swine is a complicated procedure that demands special attention to airway, feeding, and intravascular access. It is critical that each ancillary procedure be performed by a dedicated team familiar with relevant anatomy and protocol. Emphasis should be placed on secure skin-level fixation for all tube/lines to minimize risk for dislodgement. A reliable veterinarian team is invaluable and critical for long-term success.

Original languageEnglish (US)
Pages (from-to)2043-2050
Number of pages8
JournalJournal of Craniofacial Surgery
Volume25
Issue number6
DOIs
StatePublished - Nov 2014

Keywords

  • Ancillary procedure
  • Craniofacial
  • Craniomaxillofacial
  • Face transplant
  • Facial electromyography
  • Hickman catheter
  • Laser angiography
  • Maxillofacial transplant
  • PEG tube
  • Swine study
  • Tracheostomy
  • Translational study

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

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