The authors previously reported that serial propagation of the Justin strain of herpes simplex virus 1 [HSV 1 (Justin)] results in the generation of defective DNA molecules consisting of tandem repetitions of sequences of limited complexity. In the present study, HSV 1 DNA was cleaved with the restriction endonucleases BglII and EcoRI. The fragments were electrophoretically separated on agarose gels, transferred to nitrocellulose strips, and then hybridized with 32P labeled HSV 1 (Justin) defective DNA. The data allows one to conclude that DNA sequences contained in the repeat unit of defective DNA originate from the S segment of the wild type viral DNA molecule.
ASJC Scopus subject areas
- Insect Science