Anatomy of herpes simplex virus DNA. VI. Defective DNA originates from the S component

N. Frenkel; H. Locker; W. Batterson; G. S. Hayward; B. Roizman

doi:10.1128/jvi.20.2.527-531.1976

Anatomy of herpes simplex virus DNA. VI. Defective DNA originates from the S component

N. Frenkel, H. Locker, W. Batterson, G. S. Hayward, B. Roizman

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

The authors previously reported that serial propagation of the Justin strain of herpes simplex virus 1 [HSV 1 (Justin)] results in the generation of defective DNA molecules consisting of tandem repetitions of sequences of limited complexity. In the present study, HSV 1 DNA was cleaved with the restriction endonucleases BglII and EcoRI. The fragments were electrophoretically separated on agarose gels, transferred to nitrocellulose strips, and then hybridized with ³²P labeled HSV 1 (Justin) defective DNA. The data allows one to conclude that DNA sequences contained in the repeat unit of defective DNA originate from the S segment of the wild type viral DNA molecule.

Original language	English (US)
Pages (from-to)	527-531
Number of pages	5
Journal	Unknown Journal
Volume	20
Issue number	2
DOIs	https://doi.org/10.1128/jvi.20.2.527-531.1976
State	Published - 1976
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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10.1128/jvi.20.2.527-531.1976

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abstract = "The authors previously reported that serial propagation of the Justin strain of herpes simplex virus 1 [HSV 1 (Justin)] results in the generation of defective DNA molecules consisting of tandem repetitions of sequences of limited complexity. In the present study, HSV 1 DNA was cleaved with the restriction endonucleases BglII and EcoRI. The fragments were electrophoretically separated on agarose gels, transferred to nitrocellulose strips, and then hybridized with 32P labeled HSV 1 (Justin) defective DNA. The data allows one to conclude that DNA sequences contained in the repeat unit of defective DNA originate from the S segment of the wild type viral DNA molecule.",

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AU - Roizman, B.

PY - 1976

Y1 - 1976

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AB - The authors previously reported that serial propagation of the Justin strain of herpes simplex virus 1 [HSV 1 (Justin)] results in the generation of defective DNA molecules consisting of tandem repetitions of sequences of limited complexity. In the present study, HSV 1 DNA was cleaved with the restriction endonucleases BglII and EcoRI. The fragments were electrophoretically separated on agarose gels, transferred to nitrocellulose strips, and then hybridized with 32P labeled HSV 1 (Justin) defective DNA. The data allows one to conclude that DNA sequences contained in the repeat unit of defective DNA originate from the S segment of the wild type viral DNA molecule.

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Anatomy of herpes simplex virus DNA. VI. Defective DNA originates from the S component

Abstract

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