Anatomy of herpes simplex virus DNA. VI. Defective DNA originates from the S component

N. Frenkel, H. Locker, W. Batterson, G. S. Hayward, B. Roizman

Research output: Contribution to journalArticlepeer-review

Abstract

The authors previously reported that serial propagation of the Justin strain of herpes simplex virus 1 [HSV 1 (Justin)] results in the generation of defective DNA molecules consisting of tandem repetitions of sequences of limited complexity. In the present study, HSV 1 DNA was cleaved with the restriction endonucleases BglII and EcoRI. The fragments were electrophoretically separated on agarose gels, transferred to nitrocellulose strips, and then hybridized with 32P labeled HSV 1 (Justin) defective DNA. The data allows one to conclude that DNA sequences contained in the repeat unit of defective DNA originate from the S segment of the wild type viral DNA molecule.

Original languageEnglish (US)
Pages (from-to)527-531
Number of pages5
JournalUnknown Journal
Volume20
Issue number2
DOIs
StatePublished - 1976
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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