Analyzing Single Giant Unilamellar Vesicles with a Slotline-Based RF Nanometer Sensor

Yan Cui, Anne K. Kenworthy, Michael Edidin, Ralu Divan, Daniel Rosenmann, Pingshan Wang

Research output: Contribution to journalArticle

Abstract

Novel techniques that enable reagent free detection and analysis of single cells are of great interest for the development of biological and medical sciences, as well as point-of-care health service technologies. Highly sensitive and broadband RF sensors are promising candidates for such a technique. In this work, we present a highly sensitive and tunable RF sensor, which is based on interference processes and built with a 100-nm slotline structure. The highly concentrated RF fields, up to ∼ 1.76 × 107 V/m, enable strong interactions between giant unilamellar vesicles (GUVs) and fields for high-sensitivity operations. We also provide two modeling approaches to extract cell dielectric properties from measured scattering parameters. GUVs of different molecular compositions are synthesized and analyzed with the RF sensor at ∼ 2, ∼ 2.5, and ∼ 2.8 GHz with an initial |S21|min of ∼-100 dB. Corresponding GUV dielectric properties are obtained. A one-dimensional scanning of single GUV is also demonstrated.

Original languageEnglish (US)
Article number7432040
Pages (from-to)1339-1347
Number of pages9
JournalIEEE Transactions on Microwave Theory and Techniques
Volume64
Issue number4
DOIs
StatePublished - Apr 2016

Keywords

  • Complex dielectric permittivity
  • Microwave sensor
  • conformal mapping
  • giant unilamellar vesicles (GUVs)
  • microfluidics

ASJC Scopus subject areas

  • Radiation
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

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