Abstract
Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) measurements may be clinically useful in colorectal cancer detection and management, cardiac disease risk assessment, and in liver fibrosis monitoring. The analytical performance for an automated ARCHITECT® TIMP-1 immunoassay was evaluated, including precision, on-board reagent stability, effect of interfering substances, specimen collection and handling, analytical sensitivity, linearity, recovery, manual vs. autodilution, high-dose hook effect, and reagent lot-to-lot agreement. Precision was evaluated over 20 days across 2 test sites, 3 instruments, and 3 reagent lots. Total concentration CVs for a plasma panel and for low, medium, and high controls were 2.3, 2.8, 3.2, and 5.3%, respectively. No interferences were observed for other TIMPs, common interferences, and chemotherapeutic compounds. TIMP-1 results in plasma from EDTA, heparin, and plasma separator tubes showed no significant difference (
| Original language | English (US) |
|---|---|
| Pages (from-to) | 70-76 |
| Number of pages | 7 |
| Journal | Journal of Clinical Ligand Assay |
| Volume | 30 |
| Issue number | 3-4 |
| State | Published - Sep 2007 |
Fingerprint
Keywords
- Biomarker
- Colorectal cancer
- Immunoassay development
- TIMP-1
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical
Cite this
Analytical performance of an automated immunoassay for tissue inhibitor of me talloproteinases-1 (TIMP-1). / Davis, Gerard J.; Hemken, Philip M.; Dunn, Willard; Chan, Sabrina S.; Dua, Renu; Sokoll, Lori J; Dowell, Barry L.; Biegalski, Thomas T.; Elliott, Debra; Chan, Daniel Wan-Yui.
In: Journal of Clinical Ligand Assay, Vol. 30, No. 3-4, 09.2007, p. 70-76.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analytical performance of an automated immunoassay for tissue inhibitor of me talloproteinases-1 (TIMP-1)
AU - Davis, Gerard J.
AU - Hemken, Philip M.
AU - Dunn, Willard
AU - Chan, Sabrina S.
AU - Dua, Renu
AU - Sokoll, Lori J
AU - Dowell, Barry L.
AU - Biegalski, Thomas T.
AU - Elliott, Debra
AU - Chan, Daniel Wan-Yui
PY - 2007/9
Y1 - 2007/9
N2 - Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) measurements may be clinically useful in colorectal cancer detection and management, cardiac disease risk assessment, and in liver fibrosis monitoring. The analytical performance for an automated ARCHITECT® TIMP-1 immunoassay was evaluated, including precision, on-board reagent stability, effect of interfering substances, specimen collection and handling, analytical sensitivity, linearity, recovery, manual vs. autodilution, high-dose hook effect, and reagent lot-to-lot agreement. Precision was evaluated over 20 days across 2 test sites, 3 instruments, and 3 reagent lots. Total concentration CVs for a plasma panel and for low, medium, and high controls were 2.3, 2.8, 3.2, and 5.3%, respectively. No interferences were observed for other TIMPs, common interferences, and chemotherapeutic compounds. TIMP-1 results in plasma from EDTA, heparin, and plasma separator tubes showed no significant difference (
AB - Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) measurements may be clinically useful in colorectal cancer detection and management, cardiac disease risk assessment, and in liver fibrosis monitoring. The analytical performance for an automated ARCHITECT® TIMP-1 immunoassay was evaluated, including precision, on-board reagent stability, effect of interfering substances, specimen collection and handling, analytical sensitivity, linearity, recovery, manual vs. autodilution, high-dose hook effect, and reagent lot-to-lot agreement. Precision was evaluated over 20 days across 2 test sites, 3 instruments, and 3 reagent lots. Total concentration CVs for a plasma panel and for low, medium, and high controls were 2.3, 2.8, 3.2, and 5.3%, respectively. No interferences were observed for other TIMPs, common interferences, and chemotherapeutic compounds. TIMP-1 results in plasma from EDTA, heparin, and plasma separator tubes showed no significant difference (
KW - Biomarker
KW - Colorectal cancer
KW - Immunoassay development
KW - TIMP-1
UR - http://www.scopus.com/inward/record.url?scp=63449123486&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=63449123486&partnerID=8YFLogxK
M3 - Article
VL - 30
SP - 70
EP - 76
JO - Journal of Clinical Ligand Assay
JF - Journal of Clinical Ligand Assay
SN - 1081-1672
IS - 3-4
ER -