Analytical characterization of plasma membrane-derived vesicles produced via osmotic and chemical vesiculation

Sarvenaz Sarabipour, Robin B. Chan, Bowen Zhou, Gilbert Di Paolo, Kalina Hristova

Research output: Contribution to journalArticlepeer-review

Abstract

Plasma membrane-derived vesicles are being used in biophysical and biochemical research as a simple, yet native-like model of the cellular membrane. Here we report on the characterization of vesicles produced via two different vesiculation methods from CHO and A431 cell lines. The first method is a recently developed method which utilizes chloride salts to induce osmotic vesiculation. The second is a well established chemical vesiculation method which uses DTT and formaldehyde. We show that both vesiculation methods produce vesicles which contain the lipid species previously reported in the plasma membrane of these cell lines. The two methods lead to small but statistically significant differences in two lipid species only; phosphatidylcholine (PC) and plasmalogen phosphatidylethanolamine (PEp). However, highly significant differences were observed in the degree of incorporation of a membrane receptor and in the degree of retention of soluble cytosolic proteins within the vesicles.

Original languageEnglish (US)
Pages (from-to)1591-1598
Number of pages8
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1848
Issue number7
DOIs
StatePublished - Jul 1 2015

Keywords

  • Lipid composition
  • Membrane
  • Vesicle

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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