Analysis of TRPV channel activation by stimulation of FCϵRI and MRGPR receptors in mouse peritoneal mast cells

A. Solís-López, U. Kriebs, A. Marx, S. Mannebach, W. B. Liedtke, Michael Caterina, M. Freichel, V. V. Tsvilovskyy

Research output: Contribution to journalArticle

Abstract

The activation of mast cells (MC) is part of the innate and adaptive immune responses and depends on Ca2+ entry across the plasma membrane, leading to the release of preformed inflammatory mediators by degranulation or by de novo synthesis. The calcium conducting channels of the TRPV family, known by their thermo and osmotic sensitivity, have been proposed to be involved in the MC activation in murine, rat, and human mast cell models. So far, immortalized mast cell lines and nonspecific TRPV blockers have been employed to characterize the role of TRPV channels in MC. The aim of this work was to elucidate the physiological role of TRPV channels by using primary peritoneal mast cells (PMCs), a model of connective tissue type mast cells. Our RT-PCR and NanoString analysis identified the expression of TRPV1, TRPV2, and TRPV4 channels in PMCs. For determination of the functional role of the expressed TRPV channels we performed measurements of intracellular free Ca2+ concentrations and beta-hexosaminidase release in PMCs obtained from wild type and mice deficient for corresponding TRPV1, TRPV2 and TRPV4 in response to various receptor-mediated and physical stimuli. Furthermore, substances known as activators of corresponding TRPV-channels were also tested using these assays. Our results demonstrate that TRPV1, TRPV2, and TRPV4 do not participate in activation pathways triggered by activation of the high-affinity receptors for IgE (FcϵRI), Mrgprb2 receptor, or Endothelin-1 receptor nor by heat or osmotic stimulation in mouse PMCs.

Original languageEnglish (US)
Article numbere0171366
JournalPLoS One
Volume12
Issue number2
DOIs
StatePublished - Feb 1 2017

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mast cells
Mast Cells
Chemical activation
receptors
mice
beta-N-Acetylhexosaminidases
IgE Receptors
Endothelin A Receptors
Calcium Channels
Cell membranes
Rats
Assays
Cells
Tissue
Connective Tissue Cells
calcium
beta-N-acetylhexosaminidase
endothelins
calcium channels
Adaptive Immunity

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Solís-López, A., Kriebs, U., Marx, A., Mannebach, S., Liedtke, W. B., Caterina, M., ... Tsvilovskyy, V. V. (2017). Analysis of TRPV channel activation by stimulation of FCϵRI and MRGPR receptors in mouse peritoneal mast cells. PLoS One, 12(2), [e0171366]. https://doi.org/10.1371/journal.pone.0171366

Analysis of TRPV channel activation by stimulation of FCϵRI and MRGPR receptors in mouse peritoneal mast cells. / Solís-López, A.; Kriebs, U.; Marx, A.; Mannebach, S.; Liedtke, W. B.; Caterina, Michael; Freichel, M.; Tsvilovskyy, V. V.

In: PLoS One, Vol. 12, No. 2, e0171366, 01.02.2017.

Research output: Contribution to journalArticle

Solís-López, A, Kriebs, U, Marx, A, Mannebach, S, Liedtke, WB, Caterina, M, Freichel, M & Tsvilovskyy, VV 2017, 'Analysis of TRPV channel activation by stimulation of FCϵRI and MRGPR receptors in mouse peritoneal mast cells', PLoS One, vol. 12, no. 2, e0171366. https://doi.org/10.1371/journal.pone.0171366
Solís-López, A. ; Kriebs, U. ; Marx, A. ; Mannebach, S. ; Liedtke, W. B. ; Caterina, Michael ; Freichel, M. ; Tsvilovskyy, V. V. / Analysis of TRPV channel activation by stimulation of FCϵRI and MRGPR receptors in mouse peritoneal mast cells. In: PLoS One. 2017 ; Vol. 12, No. 2.
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