Analysis of the structure of empty and peptide-loaded major histocompatibility complex molecules at the cell surface

Branimir Čatipović, Giridhar Talluri, Joseph Oh, Taiyin Wei, Xiao Min Su, Teit E. Johansen, Michael A Edidin, Jonathan P Schneck

Research output: Contribution to journalArticle

Abstract

We compared the conformation of empty and peptide-loaded class I major histocompatibility complex (MHC) molecules at the cell surface. Molecular conformations were analyzed by fluorescence resonance energy transfer (FRET) between fluorescent-labeled Fab fragments bound to the α2 domain of the MHC heavy chain and fluorescent-labeled Fab fragments bound to β2-microglobulin. No FRET was found between Fab fragments bound to empty H-2Kb, but FRET was detected when empty H-2Kb molecules were loaded with peptide. The magnitude of FRET depended on the sequence of the peptide used. The results imply that empty H-2Kb molecules are in a relatively extended conformation, and that this conformation becomes more compact when peptide is bound. These changes, which are reflected in peptide-dependent binding of monoclonal antibodies, affect the surfaces of MHC molecules available for contact with T cell receptors and hence may influence T cell-receptor recognition of MHC molecules.

Original languageEnglish (US)
Pages (from-to)1753-1761
Number of pages9
JournalJournal of Experimental Medicine
Volume180
Issue number5
DOIs
StatePublished - Nov 1 1994

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Major Histocompatibility Complex
Fluorescence Resonance Energy Transfer
Immunoglobulin Fab Fragments
Peptides
T-Cell Antigen Receptor
Molecular Conformation
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Analysis of the structure of empty and peptide-loaded major histocompatibility complex molecules at the cell surface. / Čatipović, Branimir; Talluri, Giridhar; Oh, Joseph; Wei, Taiyin; Su, Xiao Min; Johansen, Teit E.; Edidin, Michael A; Schneck, Jonathan P.

In: Journal of Experimental Medicine, Vol. 180, No. 5, 01.11.1994, p. 1753-1761.

Research output: Contribution to journalArticle

Čatipović, Branimir ; Talluri, Giridhar ; Oh, Joseph ; Wei, Taiyin ; Su, Xiao Min ; Johansen, Teit E. ; Edidin, Michael A ; Schneck, Jonathan P. / Analysis of the structure of empty and peptide-loaded major histocompatibility complex molecules at the cell surface. In: Journal of Experimental Medicine. 1994 ; Vol. 180, No. 5. pp. 1753-1761.
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AU - Su, Xiao Min

AU - Johansen, Teit E.

AU - Edidin, Michael A

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