Analysis of the Length of S‐Phase Required to Show Sister Chromatid Differential Staining

Peter E. Crossen; Eugene W. Gerner; Carl W. Bell; Jeffrey M. Trent

doi:10.1111/j.1365-2184.1986.tb00752.x

Analysis of the Length of S‐Phase Required to Show Sister Chromatid Differential Staining

Peter E. Crossen, Eugene W. Gerner, Carl W. Bell, Jeffrey M. Trent

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Abstract. In vitro studies of BrdU‐dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S‐phases that the rat embryonic fibroblasts (Rat‐1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ≤50% of the first S‐phase is necessary in order to show distinctive differential staining. the importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.

Original language	English (US)
Pages (from-to)	527-532
Number of pages	6
Journal	Cell Proliferation
Volume	19
Issue number	5
DOIs	https://doi.org/10.1111/j.1365-2184.1986.tb00752.x
State	Published - Sep 1986
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

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10.1111/j.1365-2184.1986.tb00752.x

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title = "Analysis of the Length of S‐Phase Required to Show Sister Chromatid Differential Staining",

abstract = "Abstract. In vitro studies of BrdU‐dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S‐phases that the rat embryonic fibroblasts (Rat‐1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ≤50% of the first S‐phase is necessary in order to show distinctive differential staining. the importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.",

author = "Crossen, {Peter E.} and Gerner, {Eugene W.} and Bell, {Carl W.} and Trent, {Jeffrey M.}",

year = "1986",

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T1 - Analysis of the Length of S‐Phase Required to Show Sister Chromatid Differential Staining

AU - Crossen, Peter E.

AU - Gerner, Eugene W.

AU - Bell, Carl W.

AU - Trent, Jeffrey M.

PY - 1986/9

Y1 - 1986/9

N2 - Abstract. In vitro studies of BrdU‐dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S‐phases that the rat embryonic fibroblasts (Rat‐1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ≤50% of the first S‐phase is necessary in order to show distinctive differential staining. the importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.

AB - Abstract. In vitro studies of BrdU‐dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S‐phases that the rat embryonic fibroblasts (Rat‐1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ≤50% of the first S‐phase is necessary in order to show distinctive differential staining. the importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.

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JO - Cell Proliferation

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Analysis of the Length of S‐Phase Required to Show Sister Chromatid Differential Staining

Abstract

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