Analysis of the Length of S‐Phase Required to Show Sister Chromatid Differential Staining

Peter E. Crossen, Eugene W. Gerner, Carl W. Bell, Jeffrey M. Trent

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract. In vitro studies of BrdU‐dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S‐phases that the rat embryonic fibroblasts (Rat‐1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ≤50% of the first S‐phase is necessary in order to show distinctive differential staining. the importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.

Original languageEnglish (US)
Pages (from-to)527-532
Number of pages6
JournalCell Proliferation
Volume19
Issue number5
DOIs
StatePublished - Sep 1986

ASJC Scopus subject areas

  • Cell Biology

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