Analysis of the Length of S‐Phase Required to Show Sister Chromatid Differential Staining

Peter E. Crossen, Eugene W. Gerner, Carl W. Bell, Jeffrey M. Trent

Research output: Contribution to journalArticle

Abstract

Abstract. In vitro studies of BrdU‐dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S‐phases that the rat embryonic fibroblasts (Rat‐1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ≤50% of the first S‐phase is necessary in order to show distinctive differential staining. the importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.

Original languageEnglish (US)
Pages (from-to)527-532
Number of pages6
JournalCell Proliferation
Volume19
Issue number5
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Chromatids
Staining and Labeling
Bromodeoxyuridine
Mitotic Index
Hydroxyurea
DNA Replication
Fibroblasts
Cell Proliferation
Serum

ASJC Scopus subject areas

  • Cell Biology

Cite this

Analysis of the Length of S‐Phase Required to Show Sister Chromatid Differential Staining. / Crossen, Peter E.; Gerner, Eugene W.; Bell, Carl W.; Trent, Jeffrey M.

In: Cell Proliferation, Vol. 19, No. 5, 1986, p. 527-532.

Research output: Contribution to journalArticle

Crossen, Peter E. ; Gerner, Eugene W. ; Bell, Carl W. ; Trent, Jeffrey M. / Analysis of the Length of S‐Phase Required to Show Sister Chromatid Differential Staining. In: Cell Proliferation. 1986 ; Vol. 19, No. 5. pp. 527-532.
@article{9f7edffda104418e885edb5d4f6907b2,
title = "Analysis of the Length of S‐Phase Required to Show Sister Chromatid Differential Staining",
abstract = "Abstract. In vitro studies of BrdU‐dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S‐phases that the rat embryonic fibroblasts (Rat‐1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ≤50{\%} of the first S‐phase is necessary in order to show distinctive differential staining. the importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.",
author = "Crossen, {Peter E.} and Gerner, {Eugene W.} and Bell, {Carl W.} and Trent, {Jeffrey M.}",
year = "1986",
doi = "10.1111/j.1365-2184.1986.tb00752.x",
language = "English (US)",
volume = "19",
pages = "527--532",
journal = "Cell Proliferation",
issn = "0960-7722",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Analysis of the Length of S‐Phase Required to Show Sister Chromatid Differential Staining

AU - Crossen, Peter E.

AU - Gerner, Eugene W.

AU - Bell, Carl W.

AU - Trent, Jeffrey M.

PY - 1986

Y1 - 1986

N2 - Abstract. In vitro studies of BrdU‐dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S‐phases that the rat embryonic fibroblasts (Rat‐1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ≤50% of the first S‐phase is necessary in order to show distinctive differential staining. the importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.

AB - Abstract. In vitro studies of BrdU‐dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S‐phases that the rat embryonic fibroblasts (Rat‐1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ≤50% of the first S‐phase is necessary in order to show distinctive differential staining. the importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.

UR - http://www.scopus.com/inward/record.url?scp=84990623926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84990623926&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2184.1986.tb00752.x

DO - 10.1111/j.1365-2184.1986.tb00752.x

M3 - Article

AN - SCOPUS:84990623926

VL - 19

SP - 527

EP - 532

JO - Cell Proliferation

JF - Cell Proliferation

SN - 0960-7722

IS - 5

ER -