Analysis of the length of S-phase required to show sister chromatid differential staining

P. E. Crossen; E. W. Gerner; C. W. Bell; J. M. Trent

Analysis of the length of S-phase required to show sister chromatid differential staining

P. E. Crossen, E. W. Gerner, C. W. Bell, J. M. Trent

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Abstract

In vitro studies of BrdU-dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S-phases that the rat embryonic fibroblasts (Rat-1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ~50% of the first S-phase is necessary in order to show distinctive differential staining. The importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.

Original language	English (US)
Pages (from-to)	527-532
Number of pages	6
Journal	Cell and Tissue Kinetics
Volume	19
Issue number	5
State	Published - 1986
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

Cite this

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title = "Analysis of the length of S-phase required to show sister chromatid differential staining",

abstract = "In vitro studies of BrdU-dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S-phases that the rat embryonic fibroblasts (Rat-1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ~50% of the first S-phase is necessary in order to show distinctive differential staining. The importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.",

author = "Crossen, {P. E.} and Gerner, {E. W.} and Bell, {C. W.} and Trent, {J. M.}",

year = "1986",

language = "English (US)",

volume = "19",

pages = "527--532",

journal = "Cell and Tissue Kinetics",

issn = "0008-8730",

publisher = "Wiley-Blackwell",

number = "5",

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TY - JOUR

T1 - Analysis of the length of S-phase required to show sister chromatid differential staining

AU - Crossen, P. E.

AU - Gerner, E. W.

AU - Bell, C. W.

AU - Trent, J. M.

PY - 1986

Y1 - 1986

N2 - In vitro studies of BrdU-dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S-phases that the rat embryonic fibroblasts (Rat-1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ~50% of the first S-phase is necessary in order to show distinctive differential staining. The importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.

AB - In vitro studies of BrdU-dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S-phases that the rat embryonic fibroblasts (Rat-1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ~50% of the first S-phase is necessary in order to show distinctive differential staining. The importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.

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M3 - Article

C2 - 2431782

SN - 0008-8730

VL - 19

SP - 527

EP - 532

JO - Cell and Tissue Kinetics

JF - Cell and Tissue Kinetics

IS - 5

ER -

Analysis of the length of S-phase required to show sister chromatid differential staining

Abstract

ASJC Scopus subject areas

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