Analysis of the length of S-phase required to show sister chromatid differential staining

P. E. Crossen, E. W. Gerner, C. W. Bell, J. M. Trent

Research output: Contribution to journalArticle

Abstract

In vitro studies of BrdU-dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S-phases that the rat embryonic fibroblasts (Rat-1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ~50% of the first S-phase is necessary in order to show distinctive differential staining. The importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.

Original languageEnglish (US)
Pages (from-to)527-532
Number of pages6
JournalCell and Tissue Kinetics
Volume19
Issue number5
StatePublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Crossen, P. E., Gerner, E. W., Bell, C. W., & Trent, J. M. (1986). Analysis of the length of S-phase required to show sister chromatid differential staining. Cell and Tissue Kinetics, 19(5), 527-532.