Analysis of the length of S-phase required to show sister chromatid differential staining

P. E. Crossen, E. W. Gerner, C. W. Bell, J. M. Trent

Research output: Contribution to journalArticle

Abstract

In vitro studies of BrdU-dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S-phases that the rat embryonic fibroblasts (Rat-1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ~50% of the first S-phase is necessary in order to show distinctive differential staining. The importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.

Original languageEnglish (US)
Pages (from-to)527-532
Number of pages6
JournalCell and Tissue Kinetics
Volume19
Issue number5
StatePublished - 1986
Externally publishedYes

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Chromatids
S Phase
Bromodeoxyuridine
Staining and Labeling
Mitotic Index
Hydroxyurea
DNA Replication
Fibroblasts
Cell Proliferation
Serum

ASJC Scopus subject areas

  • Cell Biology

Cite this

Crossen, P. E., Gerner, E. W., Bell, C. W., & Trent, J. M. (1986). Analysis of the length of S-phase required to show sister chromatid differential staining. Cell and Tissue Kinetics, 19(5), 527-532.

Analysis of the length of S-phase required to show sister chromatid differential staining. / Crossen, P. E.; Gerner, E. W.; Bell, C. W.; Trent, J. M.

In: Cell and Tissue Kinetics, Vol. 19, No. 5, 1986, p. 527-532.

Research output: Contribution to journalArticle

Crossen, PE, Gerner, EW, Bell, CW & Trent, JM 1986, 'Analysis of the length of S-phase required to show sister chromatid differential staining', Cell and Tissue Kinetics, vol. 19, no. 5, pp. 527-532.
Crossen, P. E. ; Gerner, E. W. ; Bell, C. W. ; Trent, J. M. / Analysis of the length of S-phase required to show sister chromatid differential staining. In: Cell and Tissue Kinetics. 1986 ; Vol. 19, No. 5. pp. 527-532.
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