Analysis of the gB promoter of herpes simplex virus type 1

High-level expression requires both an 89-base-pair promoter fragment and a nontranslated leader sequence

Nels E. Pederson, Stanley Person, Fred L. Homa

Research output: Contribution to journalArticle

Abstract

To investigate the cis-acting sequences involved in regulation of a herpes simplex virus γ1 gene, deletion analyses of the glycoprotein B (gB) gene promoter were performed. In transfection assays with gB-chloramphenicol acetyltransferase plasmids, high-level constitutive expression from the gB promoter was found with an 89-bp sequence (-69 to +20). Additional sequences in the 5′-transcribed noncoding leader region (+20 to +136) were required for full stimulation by herpes simplex virus infection. Plasmids with progressive deletions of the gB leader sequence demonstrated that chloramphenicol acetyltransferease expression in infected cells was proportional to the length of the leader region retained. In recombinant viruses containing a gB-gC gene fusion, a similar 83-bp (-60 to +23) region of the gB gene was found to promote accurately initiated gC mRNA from the viral genome with the same kinetics as the wild-type gB gene. Although the kinetics of expression remained the same, RNA abundance was greater with a 298-bp (-260 to +38) promoter than with the 83-bp promoter.

Original languageEnglish (US)
Pages (from-to)6226-6232
Number of pages7
JournalJournal of Virology
Volume66
Issue number10
StatePublished - Oct 1992
Externally publishedYes

Fingerprint

Human herpesvirus 1
Human Herpesvirus 1
Base Pairing
glycoproteins
Glycoproteins
promoter regions
plasmids
Plasmids
chloramphenicol acetyltransferase
Genes
kinetics
herpes simplex
viruses
Chloramphenicol O-Acetyltransferase
genes
gene fusion
gene deletion
Viral Genome
Gene Fusion
Gene Deletion

ASJC Scopus subject areas

  • Immunology

Cite this

Analysis of the gB promoter of herpes simplex virus type 1 : High-level expression requires both an 89-base-pair promoter fragment and a nontranslated leader sequence. / Pederson, Nels E.; Person, Stanley; Homa, Fred L.

In: Journal of Virology, Vol. 66, No. 10, 10.1992, p. 6226-6232.

Research output: Contribution to journalArticle

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