Analysis of the cytotoxic effects of light-exposed hepes-containing culture medium

J. S. Zigler, J. L. Lepe-Zuniga, B. Vistica, I. Gery

Research output: Contribution to journalArticlepeer-review

Abstract

The addition of N-2-hydroxyethylpiperazine- N′-2-ethanesulfonic acid (HEPES) to RPMI 1640 medium markedly increases the production of cytotoxic products during exposure of the medium to visible light. The cytotoxicity has been analyzed by measuring uptake of [3H]thymidine by murine thymocytes cultured in preirradiated medium containing 25 m M HEPES. Complete inhibition of thymidine uptake was produced by exposing 50% of the culture medium to light for 3 h before addition of cells. The HEPES-mediated effect requires only that HEPES and riboflavin be exposed to light; other medium constituents are not necessary. Hydrogen peroxide is a principal cytotoxic agent produced in this system. It is demonstrated that most, but not all, of the inhibition of thymidine uptake can be attributed to hydrogen peroxide.

Original languageEnglish (US)
Pages (from-to)282-287
Number of pages6
JournalIn Vitro Cellular & Developmental Biology
Volume21
Issue number5
DOIs
StatePublished - May 1985

Keywords

  • HEPES
  • hydrogen peroxide
  • light-induced cytotoxicity
  • tissue culture medium

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

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