Analysis of the circulating tumor cell capture ability of a slit filter-based method in comparison to a selection-free method in multiple cancer types

Hidenori Takagi, Liang Dong, Morgan D. Kuczler, Kara Lombardo, Mitsuharu Hirai, Sarah R. Amend, Kenneth J. Pienta

Research output: Contribution to journalArticlepeer-review

Abstract

Circulating tumor cells (CTCs) are a promising biomarker for cancer liquid biopsy. To evaluate the CTC capture bias and detection capability of the slit filter-based CTC isolation platform (CTC-FIND), we prospectively compared it head to head to a selection-free platform (AccuCyte®-CyteFinder® system). We used the two methods to determine the CTC counts, CTC positive rates, CTC size distributions, and CTC phenotypes in 36 patients with metastatic cancer. Between the two methods, the median CTC counts were not significantly different and the total counts were correlated (r = 0.63, p < 0.0001). The CTC positive rate by CTC-FIND was significantly higher than that by AccuCyte®-CyteFinder® system (91.7% vs. 66.7%, p < 0.05). The median diameter of CTCs collected by CTC-FIND was significantly larger (13.0 μm, range 5.2–52.0 vs. 10.4 μm, range 5.2–44.2, p < 0.0001). The distributions of CTC phenotypes (CK+EpCAM+, CK+EpCAM− or CK−EpCAM+) detected by both methods were similar. These results suggested that CTC-FIND can detect more CTC-positive cases but with a bias toward large size of CTCs.

Original languageEnglish (US)
Article number9031
Pages (from-to)1-17
Number of pages17
JournalInternational journal of molecular sciences
Volume21
Issue number23
DOIs
StatePublished - Dec 1 2020

Keywords

  • Bladder cancer
  • Circulating tumor cells
  • Kidney cancer
  • Pancreatic cancer
  • Prostate cancer
  • Selection-free platform
  • Size-based filter

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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