Analysis of the Anopheles gambiae genome using RAPD markers

G. Favia, G. Dimopouios, C. Louis

Research output: Contribution to journalArticlepeer-review

Abstract

RAPD analysis technique is used as a rapid and reliable tool for genome analysis in the malaria vector Anopheles gambiae. Using more than eighty different commercially available primers we identified more than sixty different DNA segments that were differentially amplified in different strains of An. gambiae s.s. and An. arabiensis. An estimate of the cytogenetic position of these markers is provided by their hybridization to divisional dot‐blot filters. Potentially useful RAPD markers can be cytogenetically mapped with more precision by in situ hybridization and, as they segregate as dominant markers in a Mendelian fashion, they can also be genetically mapped relative to other genes or rearrangements. Finally, we identified markers for their potential use in the identification of different mosquito strains.

Original languageEnglish (US)
Pages (from-to)149-157
Number of pages9
JournalInsect molecular biology
Volume3
Issue number3
DOIs
StatePublished - Aug 1994
Externally publishedYes

Keywords

  • PCR amplification
  • malaria
  • repetitive DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

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