Analysis of the acetylcholine action on the electrical activities of pancreatic islet B-cells in mice

F. Tao, Q. F. Su, J. R. Zhang

Research output: Contribution to journalArticlepeer-review

Abstract

Intracellular potential recording and extracellular microiontophoretic techniques were used to study the acetylcholine (ACh) action on the electrical activities of pancreatic islet B-cells in mice. The B cell membrane potential was decreased (5-10 mV) and the spikes were increased (11-17/30 s) by the ACh microinotophoresis. These effects were dependent on glucose and completely blocked by atropine. However, pirenzepine could attenuate the electrical activity by approximately 70%. The ACh-induced membrane depolarization was Ca2+-independent and blocked by TTX. But the effect of ACh on spikes was Ca2+-dependent and not blocked by verapamil. The results showed that ACh action in the enhancement of glucose-dependent electrical activities of B-cell were mediated by muscarinic receptor, mainly by subtype M1. Then TTX-sensitive Na channel and verapamil-insensitive Ca channel were activated by M1 receptors. Na+ influx resulted in membrane depolarization and Ca2+ influx enhanced spike discharges.

Original languageEnglish (US)
Pages (from-to)105-111
Number of pages7
JournalActa Physiologica Sinica
Volume46
Issue number2
StatePublished - Jan 1 1994

Keywords

  • acetylcholine
  • intracellular potential recording
  • microiontophoresis
  • pancreatic B cells

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint

Dive into the research topics of 'Analysis of the acetylcholine action on the electrical activities of pancreatic islet B-cells in mice'. Together they form a unique fingerprint.

Cite this