Analysis of T-cell receptor gamma chain expression in the thymus.

D. M. Pardoll, A. M. Lew, W. L. Maloy, B. J. Fowlkes, A. Kruisbeek, J. A. Bluestone, R. H. Schwartz, J. E. Coligan

Research output: Contribution to journalArticlepeer-review

Abstract

While much is known about the structure and function of the T-cell receptor (TCR) alpha beta heterodimer, information has just begun to emerge about the gamma protein, the product of a third rearranging T-cell receptor gene. We describe the use of antiserum to a carboxy-terminal peptide common to several of the murine gamma chain constant regions and a monoclonal antibody to the murine T3 complex to identify products of this T-cell receptor gene family in a subpopulation of Lyt2-, L3T4- thymocytes. The gamma chain has a molecular weight of 35kD, is disulfide bonded to a 45kD partner termed delta (delta) and is associated with the T3 complex. The cells that bear this second T-cell receptor appear to represent a distinct lineage differentiating within the thymus.

Original languageEnglish (US)
Pages (from-to)241-246
Number of pages6
JournalAdvances in experimental medicine and biology
Volume225
DOIs
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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