Analysis of selectin binding to immobilized glycosphingolipids under flow conditions

Research output: Contribution to journalConference articlepeer-review

Abstract

Selectins are cell-surface proteins capable of mediating tethering and rolling of leukocytes to the inflamed vessel wall. Further evidence that glycosphingolipids constitute major ligands for E- but not P-selectin was studied. CHO-E cells tethered extensively and formed slow rolling interactions with the immobilized glycophingolipid at 1 dyn/cm2. The step size between successive bond releases from the 2,3-sLex-ceramide conjugate was estimated using a stochastic model of cell rolling. Controlled detachment assays further revealed-much stronger adhesive interactions of E- than P-selectin with 2,3-sLex glycosphingolipids.

Original languageEnglish (US)
Pages (from-to)S-88
JournalAnnals of biomedical engineering
Volume28
Issue numberSUPPL. 1
StatePublished - Dec 1 2000
Event2000 Annual Fall Meeting of the Biomedical Engineering Society - Washington, WA, USA
Duration: Oct 12 2000Oct 14 2000

ASJC Scopus subject areas

  • Biomedical Engineering

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