Analysis of selectin binding to immobilized glycosphingolipids under flow conditions

Monica Burdick, K Konstantopoulos

Research output: Contribution to journalArticle

Abstract

Selectins are cell-surface proteins capable of mediating tethering and rolling of leukocytes to the inflamed vessel wall. Further evidence that glycosphingolipids constitute major ligands for E- but not P-selectin was studied. CHO-E cells tethered extensively and formed slow rolling interactions with the immobilized glycophingolipid at 1 dyn/cm2. The step size between successive bond releases from the 2,3-sLex-ceramide conjugate was estimated using a stochastic model of cell rolling. Controlled detachment assays further revealed-much stronger adhesive interactions of E- than P-selectin with 2,3-sLex glycosphingolipids.

Original languageEnglish (US)
JournalAnnals of Biomedical Engineering
Volume28
Issue numberSUPPL. 1
StatePublished - 2000

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Ceramides
Stochastic models
Assays
Adhesives
Ligands
Proteins
Selectins

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Analysis of selectin binding to immobilized glycosphingolipids under flow conditions. / Burdick, Monica; Konstantopoulos, K.

In: Annals of Biomedical Engineering, Vol. 28, No. SUPPL. 1, 2000.

Research output: Contribution to journalArticle

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