Analysis of poly(amidoamine)-succinamic acid dendrimers by slab-gel electrophoresis and capillary zone electrophoresis

Xiangyang Shi, Anil K. Patri, Wojciech Lesniak, Mohammad T. Islam, Chunxin Zhang, James R. Baker, Lajos P. Balogh

Research output: Contribution to journalArticlepeer-review

Abstract

Ethylenediamine (EDA)-core poly(amidoamine) (PAMAM) succinamic acid dendrimers (Ex.SAH, where × refers to the generation) were synthesized and analyzed by polyacrylamide gel electrophoresis (PAGE), size-exclusion chromatography (SEC), potentiometric acid-base titration, and capillary zone electrophoresis (CZE). Various generations (E1.SAH-E7.SAH) PAMAMs and a succinamic acid terminated core-shell tecto(dendrimer) (E5(E3.SAH)n) were first analyzed by PAGE. PAGE results show that the relative mobilities of generation 2 to generation 7 dendrimers decreased with the increasing number of generations. The molecular mass of a generation 5 core generation 3 shell tecto(dendrimer) (denoted as E5(E3.SAH)n) was determined to be between the Mw of E6.SAH and E7.SAH. CZE analysis allowed the evaluation of electrophoretic properties of given-generation dendrimers. The electrophoretic mobilities of individual generations PAMAM polyanions are similar, indicating that the separation mainly depends on their approximately identical charge/mass ratio. The E5(E3.SAH)n tectodendrimer had a lower electrophoretic mobility, which was consistent with its lower charge/mass ratio. The combination of PAGE and CZE analysis provides an alternative and effective way to characterize this group of PAMAM-succinamic acid dendrimers.

Original languageEnglish (US)
Pages (from-to)2960-2967
Number of pages8
JournalElectrophoresis
Volume26
Issue number15
DOIs
StatePublished - Aug 1 2005
Externally publishedYes

Keywords

  • Capillary electrophoresis
  • Gel electrophoresis
  • Poly (amidoamine) dendrimers
  • Poly(amidoamine)-succinamic acid dendrimers
  • Polyanionic dendrimers

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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