Analysis of pol integrase sequences in diverse HIV type 1 strains using a prototype genotyping assay

Susan H. Eshleman, Sarah E. Hudelson, Peter Smith, John Hackett, Vera Holzmayer, Priscilla Swanson, Sushil G. Devare, Natalia Marlowe

Research output: Contribution to journalArticle

Abstract

A prototype assay was used to genotype integrase (IN) from 120 HIV-1- infected IN inhibitor-naive adults from Argentina, Brazil, Cameroon, South Africa, Thailand, and Uganda. Subtype designations based on analysis of pol IN sequences were A (14), B (15), C (12), D (11), F (12), G (7), H (1), CRF01-AE (9), CRF02-AG (34), CRF22-01 A1 (4), and CRF37-cpx (1). Ten (8.3%) of 120 samples had mutations associated with reduced susceptibility to the IN inhibitors, raltegravir and elvitegravir. Two samples had E92Q (both subtype B) and eight had E157Q (2A, 1C, 1D, 1F, 3 CRF02-AG). Some samples had other mutations selected by these drugs including T97A, and some had amino acid polymorphisms at positions associated with raltegravir and elvitegravir resistance. Mutations associated with other investigational HIV IN inhibitors were also identified. This suggests that HIV strains may vary in their natural susceptibility to HIV IN inhibitors.

Original languageEnglish (US)
Pages (from-to)343-345
Number of pages3
JournalAIDS research and human retroviruses
Volume25
Issue number3
DOIs
StatePublished - Mar 1 2009

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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    Eshleman, S. H., Hudelson, S. E., Smith, P., Hackett, J., Holzmayer, V., Swanson, P., Devare, S. G., & Marlowe, N. (2009). Analysis of pol integrase sequences in diverse HIV type 1 strains using a prototype genotyping assay. AIDS research and human retroviruses, 25(3), 343-345. https://doi.org/10.1089/aid.2008.0236