Analysis of Phenotype

Cory F. Brayton, Colin McKerlie, Steve Brown

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Phenotyping of genetically engineered animals is done to provide data and insight about the functions of genes. In academic hypothesis-driven research settings, phenotyping tests are limited to those that answer-specific questions. In large-scale omics (phenomics) programs like the International Mouse Phenotyping Consortium (IMPC), initial or primary phenotyping aims to be unbiased and broadly informative, systematic and standardized, subject to quality assurance (QA) and quality control (QC), and responsive to ongoing assessment and revision. Genetically engineered mice (GEM) or mouse embryonic stem (ES) cell lines, protocols, and data are publicly accessible from these international programs. These resources need to be user-friendly to optimize the utility of GEM models of human disease in translational research. In phenotyping and in other translational research involving animals, pathology is a critical aid to diagnose health problems that can compromise the research, and also is a powerful tool to identify, confirm, and characterize phenotypes, validate translational models, and provide biologically-relevant morphological data in the context of the whole animal. This chapter discusses phenotyping sensu latu, but emphasizes IMPC's 10-year plan to phenotype every protein-coding gene, as well as to develop practical pathology to support phenotyping and other translational research.

Original languageEnglish (US)
Title of host publicationTransgenic Animal Technology
Subtitle of host publicationA Laboratory Handbook: Third Edition
PublisherElsevier Inc.
Pages431-487
Number of pages57
ISBN (Print)9780124104907
DOIs
StatePublished - Jun 23 2014

Keywords

  • Genetically engineered mice (GEM)
  • International Knockout Mouse Consortium (IKMC)
  • International Mouse Phenotyping Consortium (IMPC)
  • Mice
  • Pathology
  • Phenotype
  • Phenotyping
  • Transgenic

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Analysis of Phenotype'. Together they form a unique fingerprint.

  • Cite this

    Brayton, C. F., McKerlie, C., & Brown, S. (2014). Analysis of Phenotype. In Transgenic Animal Technology: A Laboratory Handbook: Third Edition (pp. 431-487). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-410490-7.00016-5