Analysis of paired serum, urine and filter paper blood specimens for presence of filarial antigen by immunoradiometric assay

G. B.K.S. Prasad, B. C. Harinath, R. G. Hamilton

Research output: Contribution to journalArticlepeer-review

Abstract

Paired serum, urine, and finger-prick whole blood dried on filter paper were analyzed by immunoradiometric assay (IRMA) for filarial antigen using Brugia malayi-specific rabbit antibody. Nine sera and 6 urines from the 10 paired serum-urine samples obtained from individuals with microfilaremia contained IRMA detectable filarial antigen. In contrast, all serum and urine specimens from patients with chronic infections, endemic and non-endemic controls were negative. Whole blood eluted from filter paper spots contained IRMA detectable material; their degree of positivity agreed well with IRMA binding levels obtained with paired urines. Reduced recovery of antigen dried on filter paper was observed at antigen levels <10 ng/ml equivalents, presumably due to irreversible absorption onto the filter paper. Urine and finger-prick filter paper blood specimens can be used in the diagnosis of microfilaremic infections that have been associated with circulating antigen in the blood.

Original languageEnglish (US)
Pages (from-to)351-365
Number of pages15
JournalJournal of Immunoassay
Volume8
Issue number4
DOIs
StatePublished - Dec 1 1987
Externally publishedYes

Keywords

  • Filariasis
  • diagnosis
  • filarial antigens
  • filter paper blood
  • immunoradiometric assay
  • urine

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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