Analysis of microarray data using Z score transformation

Chris Cheadle, Marquis P. Vawter, William J. Freed, Kevin G. Becker

Research output: Contribution to journalArticle

Abstract

High-throughput cDNA microarray technology allows for the simultaneous analysis of gene expression levels for thousands of genes and as such, rapid, relatively simple methods are needed to store, analyze, and cross-compare basic microarray data. The application of a classical method of data normalization, Z score transformation, provides a way of standardizing data across a wide range of experiments and allows the comparison of microarray data independent of the original hybridization intensities. Data normalized by Z score transformation can be used directly in the calculation of significant changes in gene expression between different samples and conditions. We used Z scores to compare several different methods for predicting significant changes in gene expression including fold changes, Z ratios, Z and t statistical tests. We conclude that the Z score transformation normalization method accompanied by either Z ratios or Z tests for significance estimates offers a useful method for the basic analysis of microarray data. The results provided by these methods can be as rigorous and are no more arbitrary than other test methods, and, in addition, they have the advantage that they can be easily adapted to standard spreadsheet programs.

Original languageEnglish (US)
Pages (from-to)73-81
Number of pages9
JournalJournal of Molecular Diagnostics
Volume5
Issue number2
StatePublished - May 2003
Externally publishedYes

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Microarray Analysis
Gene Expression
Oligonucleotide Array Sequence Analysis
Technology
Genes

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Cheadle, C., Vawter, M. P., Freed, W. J., & Becker, K. G. (2003). Analysis of microarray data using Z score transformation. Journal of Molecular Diagnostics, 5(2), 73-81.

Analysis of microarray data using Z score transformation. / Cheadle, Chris; Vawter, Marquis P.; Freed, William J.; Becker, Kevin G.

In: Journal of Molecular Diagnostics, Vol. 5, No. 2, 05.2003, p. 73-81.

Research output: Contribution to journalArticle

Cheadle, C, Vawter, MP, Freed, WJ & Becker, KG 2003, 'Analysis of microarray data using Z score transformation', Journal of Molecular Diagnostics, vol. 5, no. 2, pp. 73-81.
Cheadle C, Vawter MP, Freed WJ, Becker KG. Analysis of microarray data using Z score transformation. Journal of Molecular Diagnostics. 2003 May;5(2):73-81.
Cheadle, Chris ; Vawter, Marquis P. ; Freed, William J. ; Becker, Kevin G. / Analysis of microarray data using Z score transformation. In: Journal of Molecular Diagnostics. 2003 ; Vol. 5, No. 2. pp. 73-81.
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