Analysis of message expression in single neurons of Alzheimer's disease brain

L. M. Callahan, N. Chow, J. E. Cheetham, Christopher Cox, P. D. Coleman

Research output: Contribution to journalArticle

Abstract

Because many cell types and disease states exist in the sample of cells in even a very small region of Alzheimer's disease (AD) brain tissue, optimal understanding of disease mechanisms requires study at the level of the single cell. Our Golgi studies of single neurons in the AD brain have revealed reduced dendritic extent in many but not all, brain regions. This reduced dendritic extent is interpreted as reduced capacity of neurons in AD to proliferate new dendritic material. Studies of message expression in single neurons reveal that neurons containing neurofibrillary tangles (NFTs) show reduced expression of messages for proteins related to growth of neuronal processes and to synapses. Neighboring neurons free of NFTs express these messages at levels approximating the levels expressed by single neurons from control brain. This reduction of expression of messages related to growth of neuronal processes and to synapses is selective, because expression of message for the lysosomal enzyme, cathepsin D, is increased in neurons containing NFTs. Simultaneous analysis of the expression of multiple genes by single neurons using an aRNA technique offers powerful capacity to profile message expression as a function of disease state of single cells.

Original languageEnglish (US)
JournalNeurobiology of Aging
Volume19
Issue numberSUPPL. 1
DOIs
StatePublished - Jan 1998
Externally publishedYes

Fingerprint

Alzheimer Disease
Neurons
Brain
Neurofibrillary Tangles
Synapses
Cathepsin D
Growth
Gene Expression
Enzymes
Proteins

Keywords

  • Alzheimer's disease
  • Message
  • Single neurons

ASJC Scopus subject areas

  • Clinical Neurology
  • Biological Psychiatry
  • Developmental Neuroscience
  • Neurology
  • Psychology(all)

Cite this

Analysis of message expression in single neurons of Alzheimer's disease brain. / Callahan, L. M.; Chow, N.; Cheetham, J. E.; Cox, Christopher; Coleman, P. D.

In: Neurobiology of Aging, Vol. 19, No. SUPPL. 1, 01.1998.

Research output: Contribution to journalArticle

Callahan, L. M. ; Chow, N. ; Cheetham, J. E. ; Cox, Christopher ; Coleman, P. D. / Analysis of message expression in single neurons of Alzheimer's disease brain. In: Neurobiology of Aging. 1998 ; Vol. 19, No. SUPPL. 1.
@article{cebf4e3fbb514a79ad929787e96b6c33,
title = "Analysis of message expression in single neurons of Alzheimer's disease brain",
abstract = "Because many cell types and disease states exist in the sample of cells in even a very small region of Alzheimer's disease (AD) brain tissue, optimal understanding of disease mechanisms requires study at the level of the single cell. Our Golgi studies of single neurons in the AD brain have revealed reduced dendritic extent in many but not all, brain regions. This reduced dendritic extent is interpreted as reduced capacity of neurons in AD to proliferate new dendritic material. Studies of message expression in single neurons reveal that neurons containing neurofibrillary tangles (NFTs) show reduced expression of messages for proteins related to growth of neuronal processes and to synapses. Neighboring neurons free of NFTs express these messages at levels approximating the levels expressed by single neurons from control brain. This reduction of expression of messages related to growth of neuronal processes and to synapses is selective, because expression of message for the lysosomal enzyme, cathepsin D, is increased in neurons containing NFTs. Simultaneous analysis of the expression of multiple genes by single neurons using an aRNA technique offers powerful capacity to profile message expression as a function of disease state of single cells.",
keywords = "Alzheimer's disease, Message, Single neurons",
author = "Callahan, {L. M.} and N. Chow and Cheetham, {J. E.} and Christopher Cox and Coleman, {P. D.}",
year = "1998",
month = "1",
doi = "10.1016/S0197-4580(98)00044-X",
language = "English (US)",
volume = "19",
journal = "Neurobiology of Aging",
issn = "0197-4580",
publisher = "Elsevier Inc.",
number = "SUPPL. 1",

}

TY - JOUR

T1 - Analysis of message expression in single neurons of Alzheimer's disease brain

AU - Callahan, L. M.

AU - Chow, N.

AU - Cheetham, J. E.

AU - Cox, Christopher

AU - Coleman, P. D.

PY - 1998/1

Y1 - 1998/1

N2 - Because many cell types and disease states exist in the sample of cells in even a very small region of Alzheimer's disease (AD) brain tissue, optimal understanding of disease mechanisms requires study at the level of the single cell. Our Golgi studies of single neurons in the AD brain have revealed reduced dendritic extent in many but not all, brain regions. This reduced dendritic extent is interpreted as reduced capacity of neurons in AD to proliferate new dendritic material. Studies of message expression in single neurons reveal that neurons containing neurofibrillary tangles (NFTs) show reduced expression of messages for proteins related to growth of neuronal processes and to synapses. Neighboring neurons free of NFTs express these messages at levels approximating the levels expressed by single neurons from control brain. This reduction of expression of messages related to growth of neuronal processes and to synapses is selective, because expression of message for the lysosomal enzyme, cathepsin D, is increased in neurons containing NFTs. Simultaneous analysis of the expression of multiple genes by single neurons using an aRNA technique offers powerful capacity to profile message expression as a function of disease state of single cells.

AB - Because many cell types and disease states exist in the sample of cells in even a very small region of Alzheimer's disease (AD) brain tissue, optimal understanding of disease mechanisms requires study at the level of the single cell. Our Golgi studies of single neurons in the AD brain have revealed reduced dendritic extent in many but not all, brain regions. This reduced dendritic extent is interpreted as reduced capacity of neurons in AD to proliferate new dendritic material. Studies of message expression in single neurons reveal that neurons containing neurofibrillary tangles (NFTs) show reduced expression of messages for proteins related to growth of neuronal processes and to synapses. Neighboring neurons free of NFTs express these messages at levels approximating the levels expressed by single neurons from control brain. This reduction of expression of messages related to growth of neuronal processes and to synapses is selective, because expression of message for the lysosomal enzyme, cathepsin D, is increased in neurons containing NFTs. Simultaneous analysis of the expression of multiple genes by single neurons using an aRNA technique offers powerful capacity to profile message expression as a function of disease state of single cells.

KW - Alzheimer's disease

KW - Message

KW - Single neurons

UR - http://www.scopus.com/inward/record.url?scp=0031957978&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031957978&partnerID=8YFLogxK

U2 - 10.1016/S0197-4580(98)00044-X

DO - 10.1016/S0197-4580(98)00044-X

M3 - Article

C2 - 9562477

AN - SCOPUS:0031957978

VL - 19

JO - Neurobiology of Aging

JF - Neurobiology of Aging

SN - 0197-4580

IS - SUPPL. 1

ER -