TY - JOUR
T1 - Analysis of K-ras, N-ras, H-ras, and p53 in lung neuroendocrine neoplasms
AU - Couce, Marta Emma
AU - Bautista, Dolores
AU - Costa, Jose
AU - Carter, Darrell
PY - 1999/6
Y1 - 1999/6
N2 - This study screened 11 samples of typical carcinoid (TC), 4 samples of atypical carcinoid (AC), 1 sample of large cell neuroendocrine carcinoma (LCNEC), and four metastases for point mutations in exons 5 to 8 of the p53 gene, and exons 1 and 2 of the K-ras, H-ras, and N-ras genes using polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) and direct sequencing and by immunohistochemistry for p53. Exon 1 of K-ras was mutated in two samples of low-grade AC and a metastasis from one of these tumors (GAT12 and AGT12, respectively). No mutations in N-ras or H-ras were found. Mutations in exons 5 and 8 of the p53 gene were identified in a high- grade AC and a LCNEC. Positive immunostaining for p53 was present in three samples, with only one genotypic mutation shown (LCNEC). In conclusion, point mutations of the p53 gene were infrequent in these pulmonary neuroendocrine tumors, did not correlate in all samples with immunostaining, and were associated with the higher-grade tumors. Second, the presence of K-ras mutations seems to be associated with the higher-grade carcinomas. Third, N- ras and H-ras mutations were not found with these pulmonary neuroendocrine tumors.
AB - This study screened 11 samples of typical carcinoid (TC), 4 samples of atypical carcinoid (AC), 1 sample of large cell neuroendocrine carcinoma (LCNEC), and four metastases for point mutations in exons 5 to 8 of the p53 gene, and exons 1 and 2 of the K-ras, H-ras, and N-ras genes using polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) and direct sequencing and by immunohistochemistry for p53. Exon 1 of K-ras was mutated in two samples of low-grade AC and a metastasis from one of these tumors (GAT12 and AGT12, respectively). No mutations in N-ras or H-ras were found. Mutations in exons 5 and 8 of the p53 gene were identified in a high- grade AC and a LCNEC. Positive immunostaining for p53 was present in three samples, with only one genotypic mutation shown (LCNEC). In conclusion, point mutations of the p53 gene were infrequent in these pulmonary neuroendocrine tumors, did not correlate in all samples with immunostaining, and were associated with the higher-grade tumors. Second, the presence of K-ras mutations seems to be associated with the higher-grade carcinomas. Third, N- ras and H-ras mutations were not found with these pulmonary neuroendocrine tumors.
KW - H-ras
KW - K-ras
KW - Lung carcinoid
KW - N-ras
KW - Neuroendocrine
KW - P53
UR - http://www.scopus.com/inward/record.url?scp=0032771572&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032771572&partnerID=8YFLogxK
U2 - 10.1097/00019606-199906000-00003
DO - 10.1097/00019606-199906000-00003
M3 - Article
C2 - 10475381
AN - SCOPUS:0032771572
SN - 1052-9551
VL - 8
SP - 71
EP - 79
JO - Diagnostic Molecular Pathology
JF - Diagnostic Molecular Pathology
IS - 2
ER -