Analysis of Hypoxia-Inducible Factor 1α Expression and its Effects on Invasion and Metastasis

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Hypoxia-inducible factor 1 (HIF-1) plays an important role in human cancer cell invasion and metastasis. As a result, overexpression of the HIF-1α subunit in biopsy specimens is associated with increased patient mortality in several common cancers, including breast adenocarcinoma and oropharyngeal squamous cell carcinoma. Here, we describe methods for immunohistochemical detection of HIF-1α in tumor biopsy sections and ex vivo assays for analyzing the effects of hypoxia and HIF-1 on cancer cell invasiveness and cell-cell adhesion.

Original languageEnglish (US)
Title of host publicationOxygen Biology and Hypoxia
EditorsHelmut Sies, Bernhard Brune
Pages347-354
Number of pages8
DOIs
StatePublished - 2007

Publication series

NameMethods in Enzymology
Volume435
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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