Analysis of HIV type 1 gp41 sequences in diverse HIV type 1 strains

Susan Eshleman, Sarah E. Hudelson, Robert Bruce, Travis Lee, Michelle R. Owens, John Hackett, Priscilla Swanson, Sushil G. Devare, Natalia Marlowe

Research output: Contribution to journalArticle

Abstract

The HIV fusion inhibitor enfuvirtide (ENF/Fuzeon) targets the env gp41 transmembrane domain. Mutations in gp41 are associated with ENF resistance. We developed a prototype assay to genotype a 676-bp region spanning the heptad repeat domains (HR1 and HR2) of HIV-1 gp41. Plasma samples were collected from 126 HIV-1-infected blood donors in Cameroon, Brazil, Uganda, South Africa, Thailand, and Argentina. Based on analysis of gag p24, pol integrase, and env gp41 genes, the panel was composed of subtypes A/A2 (18), B (11), C (14), D (10), F/F2 (9), G (7), CRF01_AE (9), CRF02_AG (33), and recombinant strains (15). Genotyping was successful for 119 of the 126 samples (94.4%). Although numerous amino acid polymorphisms were detected in some samples, none had primary mutations associated with ENF resistance. The gp41 HIV-1 research reagents developed by Celera are useful tools for genotyping analysis of the gp41 region in diverse HIV-1 strains.

Original languageEnglish (US)
Pages (from-to)1593-1598
Number of pages6
JournalAIDS Research and Human Retroviruses
Volume23
Issue number12
DOIs
StatePublished - Dec 1 2007

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HIV-1
HIV Envelope Protein gp41
HIV Fusion Inhibitors
env Genes
Integrases
Cameroon
Mutation
Uganda
Argentina
Thailand
South Africa
Blood Donors
varespladib methyl
Brazil
Genotype
Amino Acids
Research
enfuvirtide

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Eshleman, S., Hudelson, S. E., Bruce, R., Lee, T., Owens, M. R., Hackett, J., ... Marlowe, N. (2007). Analysis of HIV type 1 gp41 sequences in diverse HIV type 1 strains. AIDS Research and Human Retroviruses, 23(12), 1593-1598. https://doi.org/10.1089/aid.2007.0130

Analysis of HIV type 1 gp41 sequences in diverse HIV type 1 strains. / Eshleman, Susan; Hudelson, Sarah E.; Bruce, Robert; Lee, Travis; Owens, Michelle R.; Hackett, John; Swanson, Priscilla; Devare, Sushil G.; Marlowe, Natalia.

In: AIDS Research and Human Retroviruses, Vol. 23, No. 12, 01.12.2007, p. 1593-1598.

Research output: Contribution to journalArticle

Eshleman, S, Hudelson, SE, Bruce, R, Lee, T, Owens, MR, Hackett, J, Swanson, P, Devare, SG & Marlowe, N 2007, 'Analysis of HIV type 1 gp41 sequences in diverse HIV type 1 strains', AIDS Research and Human Retroviruses, vol. 23, no. 12, pp. 1593-1598. https://doi.org/10.1089/aid.2007.0130
Eshleman, Susan ; Hudelson, Sarah E. ; Bruce, Robert ; Lee, Travis ; Owens, Michelle R. ; Hackett, John ; Swanson, Priscilla ; Devare, Sushil G. ; Marlowe, Natalia. / Analysis of HIV type 1 gp41 sequences in diverse HIV type 1 strains. In: AIDS Research and Human Retroviruses. 2007 ; Vol. 23, No. 12. pp. 1593-1598.
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