Analysis of HIV diversity using a high-resolution melting assay

William I. Towler, Maria M. James, Stuart Campbell Ray, Lei Wang, Deborah Donnell, Anthony Mwatha, Laura Guay, Clemensia Nakabiito, Philippa Musoke, J. Brooks Jackson, Susan Eshleman

Research output: Contribution to journalArticle

Abstract

HIV viruses are usually genetically homogeneous shortly after infection, and become more heterogeneous over time. We developed a high-resolution melting (HRM) assay to analyze HIV diversity without sequencing. Plasma samples from the HIVNET 012 trial were obtained from nine Ugandan mother-infant pairs. DNA amplified from the HIV gag region was analyzed to determine the number of degrees over which the DNA melted (HRM score). HRM gag DNA was also cloned and sequenced (50 clones/mother; 20 clones/infant). The median HRM score for infants (4.3, range 4.2-5.3) was higher than that for control plasmids (3.4, range 3.2-3.8, p

Original languageEnglish (US)
Pages (from-to)913-918
Number of pages6
JournalAIDS Research and Human Retroviruses
Volume26
Issue number8
DOIs
StatePublished - Aug 1 2010

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Freezing
HIV
Clone Cells
Mothers
Nucleic Acid Denaturation
DNA
Plasmids
Viruses
Infection

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases
  • Medicine(all)

Cite this

Analysis of HIV diversity using a high-resolution melting assay. / Towler, William I.; James, Maria M.; Ray, Stuart Campbell; Wang, Lei; Donnell, Deborah; Mwatha, Anthony; Guay, Laura; Nakabiito, Clemensia; Musoke, Philippa; Jackson, J. Brooks; Eshleman, Susan.

In: AIDS Research and Human Retroviruses, Vol. 26, No. 8, 01.08.2010, p. 913-918.

Research output: Contribution to journalArticle

Towler, WI, James, MM, Ray, SC, Wang, L, Donnell, D, Mwatha, A, Guay, L, Nakabiito, C, Musoke, P, Jackson, JB & Eshleman, S 2010, 'Analysis of HIV diversity using a high-resolution melting assay', AIDS Research and Human Retroviruses, vol. 26, no. 8, pp. 913-918. https://doi.org/10.1089/aid.2009.0259
Towler, William I. ; James, Maria M. ; Ray, Stuart Campbell ; Wang, Lei ; Donnell, Deborah ; Mwatha, Anthony ; Guay, Laura ; Nakabiito, Clemensia ; Musoke, Philippa ; Jackson, J. Brooks ; Eshleman, Susan. / Analysis of HIV diversity using a high-resolution melting assay. In: AIDS Research and Human Retroviruses. 2010 ; Vol. 26, No. 8. pp. 913-918.
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