Analysis of glucosinolates from broccoli and other cruciferous vegetables by hydrophilic interaction liquid chromatography

John K. Troyer, Katherine K. Stephenson, Jed W. Fahey

Research output: Contribution to journalArticle

Abstract

While methods for the identification and quantification of total plant glucosinolate content typically utilize desulfation of glucosinolates followed by reversed-phase chromatography, the analysis of intact glucosinolates has been problematic. Hydrophilic interaction chromatography offers a novel method for analyzing intact glucosinolates and when performed along with ion-pair reversed-phase chromatography offers a powerful and complementary method for glucosinolate analysis.

Original languageEnglish (US)
Pages (from-to)299-304
Number of pages6
JournalJournal of Chromatography A
Volume919
Issue number2
DOIs
StatePublished - Jun 15 2001

Keywords

  • Food analysis
  • Glucosinolates
  • Vegetables

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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