TY - JOUR
T1 - Analysis of genetic polymorphism in select vaccine candidate antigens and microsatellite loci in Plasmodium falciparum from endemic areas at varying altitudes
AU - Mlambo, Godfree
AU - Sullivan, David
AU - Mutambu, Susan L.
AU - Soko, White
AU - Mbedzi, Joel
AU - Chivenga, James
AU - Jaenisch, Thomas
AU - Gemperli, Armin
AU - Kumar, Nirbhay
N1 - Funding Information:
We would like to thank the National Institute of Health Research in Zimbabwe for facilitating sample collection. We also acknowledge the Fogarty International Center Training Grant (TW001587) for funding and training of Godfree Mlambo. Research in the Kumar and Sullivan labs is supported by research grants from the NIH.
PY - 2007/6
Y1 - 2007/6
N2 - Plasmodium falciparum parasites obtained from symptomatic patients attending clinics in Bindura (altitude 1100 m), Chiredzi (600 m) and Kariba (<600 m), previously reported to differ in malaria endemicity were genotyped on the msp-1, msp-2 and glurp loci to examine the extent of parasite genetic diversity. While the parasites were monomorphic for msp-1 allele RO33 from the three locations, the K1 allele was over-represented in Kariba (p = 0.02) and Mad20 alleles occurred at a higher frequency in Bindura. A similar PCR analysis for glurp and the two main allelic families of msp-2, i.e. IC/3D7 and FC-27 revealed minimal differences in the parasite population. A total of 8 msp-1 Block 2 and 11 msp-2 genotypes were identified from the three locations combined. On the glurp locus, 13 different genotypes ranging in size from 660 to 1160 bp were detected in parasites obtained from Bindura and Kariba. To gain further insight into P. falciparum genetic diversity in the three different geographical locations, parasites were examined for neutral microsatellite markers (C4M8, C13M30 and TA81). The number of microsatellite alleles ranged from 8 to 17 and the average expected heterozygosity (HE) for the three areas combined was 0.83 suggesting that the parasite population of Zimbabwe is genetically heterogeneous. These findings have implications in understanding the impact of genetic variation on immunity and possibly emergence of drug resistance.
AB - Plasmodium falciparum parasites obtained from symptomatic patients attending clinics in Bindura (altitude 1100 m), Chiredzi (600 m) and Kariba (<600 m), previously reported to differ in malaria endemicity were genotyped on the msp-1, msp-2 and glurp loci to examine the extent of parasite genetic diversity. While the parasites were monomorphic for msp-1 allele RO33 from the three locations, the K1 allele was over-represented in Kariba (p = 0.02) and Mad20 alleles occurred at a higher frequency in Bindura. A similar PCR analysis for glurp and the two main allelic families of msp-2, i.e. IC/3D7 and FC-27 revealed minimal differences in the parasite population. A total of 8 msp-1 Block 2 and 11 msp-2 genotypes were identified from the three locations combined. On the glurp locus, 13 different genotypes ranging in size from 660 to 1160 bp were detected in parasites obtained from Bindura and Kariba. To gain further insight into P. falciparum genetic diversity in the three different geographical locations, parasites were examined for neutral microsatellite markers (C4M8, C13M30 and TA81). The number of microsatellite alleles ranged from 8 to 17 and the average expected heterozygosity (HE) for the three areas combined was 0.83 suggesting that the parasite population of Zimbabwe is genetically heterogeneous. These findings have implications in understanding the impact of genetic variation on immunity and possibly emergence of drug resistance.
KW - Genetic polymorphism
KW - Microsatellite
KW - Plasmodium falciparum
KW - Vaccine candidate antigens
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U2 - 10.1016/j.actatropica.2007.05.001
DO - 10.1016/j.actatropica.2007.05.001
M3 - Article
C2 - 17568548
AN - SCOPUS:34347338660
SN - 0001-706X
VL - 102
SP - 201
EP - 205
JO - Acta Tropica
JF - Acta Tropica
IS - 3
ER -