Analysis of Gal4 expression patterns in adult drosophila females

Lesley N. Weaver, Tianlu Ma, Daniela Drummond-Barbosa

Research output: Contribution to journalArticlepeer-review

Abstract

Precise genetic manipulation of specific cell types or tissues to pinpoint gene function requirement is a critical step in studies aimed at unraveling the intricacies of organismal physiology. Drosophila researchers heavily rely on the UAS/Gal4/Gal80 system for tissue-specific manipulations; however, it is often unclear whether the reported Gal4 expression patterns are indeed specific to the tissue of interest such that experimental results are not confounded by secondary sites of Gal4 expression. Here, we surveyed the expression patterns of commonly used Gal4 drivers in adult Drosophila female tissues under optimal conditions and found that multiple drivers have unreported secondary sites of expression beyond their published cell type/tissue expression pattern. These results underscore the importance of thoroughly characterizing Gal4 tools as part of a rigorous experimental design that avoids potential misinterpretation of results as we strive for understanding how the function of a specific gene/pathway in one tissue contributes to whole-body physiology.

Original languageEnglish (US)
Pages (from-to)4147-4158
Number of pages12
JournalG3: Genes, Genomes, Genetics
Volume10
Issue number11
DOIs
StatePublished - Nov 2020

Keywords

  • Adult
  • Drosophila
  • Female
  • Gal4
  • Tissue-specific expression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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