Analysis of G protein γ subunit heterogeneity using mass spectrometry

Michael D. Wilcox, Kevin L. Schey, Jane Dingus, Nitin D. Mehta, Bronwyn S. Tatum, Marc Halushka, Jeffrey W. Finch, John D. Hildebrandt

Research output: Contribution to journalArticlepeer-review

Abstract

The diversity of the γ subunits in bovine brain G protein preparations was investigated using matrix-assisted laser desorption ionization (MALDI) mass spectrometry. Analysis of these G protein mixtures revealed at least four γ subunit masses by the following four criteria. 1) The measured masses were in the same mass range as the predicted molecular weights of γ isoforms. 2) The masses were reproducible between the same or different preparations of G proteins. 3) The masses were independent of the matrix used for MALDI analysis. 4) The masses comigrated with the γ subunit, as part of the heterotrimer, the βγ dimer, or the separated γ subunit. These measured masses were compared with those calculated from cDNA sequences of known bovine brain γ isoforms with the addition of plausible post-translational modifications. The mass of each spectral peak was consistent with the calculated mass for only one of four known bovine brain γ subunit isoforms, but the data suggest modifications of the γ subunits in addition to those already known or suspected at their carboxyl termini. Besides these four major masses, several additional, less resolved spectral peaks were observed whose measured masses did not correlate with any known γ subunit or plausible modification. MALDI mass spectrometry promises to be a powerful technique for the analysis of the diversity of the γ subunit in G proteins and for the characterization of their post-translational modifications.

Original languageEnglish (US)
Pages (from-to)12508-12513
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number17
StatePublished - Apr 29 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Analysis of G protein γ subunit heterogeneity using mass spectrometry'. Together they form a unique fingerprint.

Cite this