Analysis of exosome release as a cellular response to MAPK pathway inhibition

K. Agarwal, M. Saji, S. M. Lazaroff, A. F. Palmer, M. D. Ringel, Michael Paulaitis

Research output: Contribution to journalArticle

Abstract

Exosome size distributions and numbers of exosomes released per cell are measured by asymmetric flow-field flow fractionation/multi-angle light scattering (A4F/MALS) for three thyroid cancer cell lines as a function of a treatment that inhibits MAPK signaling pathways in the cells. We show that these cell lines release exosomes with well-defined morphological features and size distributions that reflect a common biological process for their formation and release into the extracellular environment. We find that those cell lines with constitutive activation of the MAPK signaling pathway display MEK-dependent exosome release characterized by increased numbers of exosomes released per cell. Analysis of the measured exosome size distributions based on a generalized extreme value distribution model for exosome formation in intracellular multivesicular bodies highlights the importance of this experimental observable for delineating different mechanisms of vesicle formation and predicting how changes in exosome release can be modified by pathway inhibitors in a cell context-dependent manner.

Original languageEnglish (US)
Pages (from-to)5440-5448
Number of pages9
JournalLangmuir
Volume31
Issue number19
DOIs
StatePublished - May 19 2015
Externally publishedYes

Fingerprint

Cells
cultured cells
cells
Mitogen-Activated Protein Kinase Kinases
Fractionation
Light scattering
Flow fields
Chemical activation
Display devices
fractionation
inhibitors
flow distribution
light scattering
cancer
activation

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Agarwal, K., Saji, M., Lazaroff, S. M., Palmer, A. F., Ringel, M. D., & Paulaitis, M. (2015). Analysis of exosome release as a cellular response to MAPK pathway inhibition. Langmuir, 31(19), 5440-5448. https://doi.org/10.1021/acs.langmuir.5b00095

Analysis of exosome release as a cellular response to MAPK pathway inhibition. / Agarwal, K.; Saji, M.; Lazaroff, S. M.; Palmer, A. F.; Ringel, M. D.; Paulaitis, Michael.

In: Langmuir, Vol. 31, No. 19, 19.05.2015, p. 5440-5448.

Research output: Contribution to journalArticle

Agarwal, K, Saji, M, Lazaroff, SM, Palmer, AF, Ringel, MD & Paulaitis, M 2015, 'Analysis of exosome release as a cellular response to MAPK pathway inhibition', Langmuir, vol. 31, no. 19, pp. 5440-5448. https://doi.org/10.1021/acs.langmuir.5b00095
Agarwal, K. ; Saji, M. ; Lazaroff, S. M. ; Palmer, A. F. ; Ringel, M. D. ; Paulaitis, Michael. / Analysis of exosome release as a cellular response to MAPK pathway inhibition. In: Langmuir. 2015 ; Vol. 31, No. 19. pp. 5440-5448.
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