Analysis of drosophila mRNA by in situ hybridization: Sequences transcribed in normal and heat shocked cultured cells

A. Spradling, S. Penman, M. L. Pardue

Research output: Contribution to journalArticlepeer-review

Abstract

Messenger RNA transcribed in cultured Drosophila cells adapted for growth under conditions permitting labeling to high specific activity has been analyzed by the technique of in situ hybridization. Poly(A)-containing cytoplasmic RNA binds specifically and reproducibly to about 50 bands in the salivary gland polytene chromosomes. In addition, heavy labeling of the β-heterochromatin associated with each of the chromosome arms is observed. The species which are detected probably belong to the more abundant classes of RNA. When the cultured Drosophila cells are subjected to heat shock immediately before labeling with 3H-uridine, there is a drastic alteration in the pattern of gene transcription detected by in situ hybridization. Most of the mRNA synthesis which could be detected in the normal cell is shut off. Newly synthesized RNA hybridizes strongly to seven new sites which do not bind mRNA from control cells. The new loci correspond almost exactly to the regions of Drosophila polytene chromosomes which puff when intact larvae are subjected to an identical heat treatment.

Original languageEnglish (US)
Pages (from-to)395-404
Number of pages10
JournalCell
Volume4
Issue number4
DOIs
StatePublished - 1975
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

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