Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India

Sivaram Gunisetty, Kaustuv Nayak, Ramesh Chandra Rai, Yadya Chawla, Elluri Seetharami Reddy, Charu Aggarwal, Deepti Maheshwari, Harekrushna Panda, Nasim Akhtar Ansari, Prabhat Singh, Manpreet Kaur, Kritika Dixit, Pragati Sharma, Priya Bhatnagar, Lalita Priyamvada, Siddhartha Kumar Bhaumik, Syed Fazil Ahamed, Rosario Vivek, Pratima Ray, Anita ShetPoonam Coshic, Rakesh Lodha, Sushil Kumar Kabra, Dil Afroze, Adfar Yousuf, Rafi Ahmed, Kaja Murali-Krishna, Anmol Chandele

Research output: Contribution to journalArticle

Abstract

Background: The Indian population is facing highest dengue burden worldwide supporting an urgent need for vaccines. For vaccine introduction, evaluation and interpretation it is important to gain a critical understanding of immune memory induced by natural exposure. However, immune memory to dengue remains poorly characterized in this region. Methods: We enumerated levels of dengue specific memory B cells (MBC), neutralizing (NT) and binding antibodies in healthy adults (n = 70) from New Delhi. Results: NT-antibodies, binding antibodies and MBC were detectable in 86%, 86.56% and 81.63% of the subjects respectively. Among the neutralizing positive subjects, 58%, 27%, 5% and 10% neutralized all four, any three, any two and any one dengue serotypes respectively. The presence of the neutralizing antibodies was associated with the presence of the MBC and binding antibodies. However, a massive interindividual variation was observed in the levels of the neutralizing antibodies (range, <1:50–1:30,264), binding antibodies (range, 1:3,000–1:134,000,) as well as the MBC (range = 0.006%–5.05%). Conclusion: These results indicate that a vast majority of the adults are immune to multiple dengue serotypes and show massive interindividual variation in neutralizing/binding antibodies and MBCs – emphasizing the importance of monitoring multiple parameters of immune memory in order to properly plan, evaluate and interpret dengue vaccines.

Original languageEnglish (US)
JournalInternational Journal of Infectious Diseases
DOIs
StatePublished - Jan 1 2019

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Dengue
Neutralizing Antibodies
India
B-Lymphocytes
Antibodies
Dengue Vaccines
Vaccines
Population

Keywords

  • Dengue
  • India
  • Memory B-cell
  • Neutralizing antibody

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India. / Gunisetty, Sivaram; Nayak, Kaustuv; Chandra Rai, Ramesh; Chawla, Yadya; Reddy, Elluri Seetharami; Aggarwal, Charu; Maheshwari, Deepti; Panda, Harekrushna; Ansari, Nasim Akhtar; Singh, Prabhat; Kaur, Manpreet; Dixit, Kritika; Sharma, Pragati; Bhatnagar, Priya; Priyamvada, Lalita; Bhaumik, Siddhartha Kumar; Ahamed, Syed Fazil; Vivek, Rosario; Ray, Pratima; Shet, Anita; Coshic, Poonam; Lodha, Rakesh; Kabra, Sushil Kumar; Afroze, Dil; Yousuf, Adfar; Ahmed, Rafi; Murali-Krishna, Kaja; Chandele, Anmol.

In: International Journal of Infectious Diseases, 01.01.2019.

Research output: Contribution to journalArticle

Gunisetty, S, Nayak, K, Chandra Rai, R, Chawla, Y, Reddy, ES, Aggarwal, C, Maheshwari, D, Panda, H, Ansari, NA, Singh, P, Kaur, M, Dixit, K, Sharma, P, Bhatnagar, P, Priyamvada, L, Bhaumik, SK, Ahamed, SF, Vivek, R, Ray, P, Shet, A, Coshic, P, Lodha, R, Kabra, SK, Afroze, D, Yousuf, A, Ahmed, R, Murali-Krishna, K & Chandele, A 2019, 'Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India', International Journal of Infectious Diseases. https://doi.org/10.1016/j.ijid.2019.01.018
Gunisetty, Sivaram ; Nayak, Kaustuv ; Chandra Rai, Ramesh ; Chawla, Yadya ; Reddy, Elluri Seetharami ; Aggarwal, Charu ; Maheshwari, Deepti ; Panda, Harekrushna ; Ansari, Nasim Akhtar ; Singh, Prabhat ; Kaur, Manpreet ; Dixit, Kritika ; Sharma, Pragati ; Bhatnagar, Priya ; Priyamvada, Lalita ; Bhaumik, Siddhartha Kumar ; Ahamed, Syed Fazil ; Vivek, Rosario ; Ray, Pratima ; Shet, Anita ; Coshic, Poonam ; Lodha, Rakesh ; Kabra, Sushil Kumar ; Afroze, Dil ; Yousuf, Adfar ; Ahmed, Rafi ; Murali-Krishna, Kaja ; Chandele, Anmol. / Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India. In: International Journal of Infectious Diseases. 2019.
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T1 - Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India

AU - Gunisetty, Sivaram

AU - Nayak, Kaustuv

AU - Chandra Rai, Ramesh

AU - Chawla, Yadya

AU - Reddy, Elluri Seetharami

AU - Aggarwal, Charu

AU - Maheshwari, Deepti

AU - Panda, Harekrushna

AU - Ansari, Nasim Akhtar

AU - Singh, Prabhat

AU - Kaur, Manpreet

AU - Dixit, Kritika

AU - Sharma, Pragati

AU - Bhatnagar, Priya

AU - Priyamvada, Lalita

AU - Bhaumik, Siddhartha Kumar

AU - Ahamed, Syed Fazil

AU - Vivek, Rosario

AU - Ray, Pratima

AU - Shet, Anita

AU - Coshic, Poonam

AU - Lodha, Rakesh

AU - Kabra, Sushil Kumar

AU - Afroze, Dil

AU - Yousuf, Adfar

AU - Ahmed, Rafi

AU - Murali-Krishna, Kaja

AU - Chandele, Anmol

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: The Indian population is facing highest dengue burden worldwide supporting an urgent need for vaccines. For vaccine introduction, evaluation and interpretation it is important to gain a critical understanding of immune memory induced by natural exposure. However, immune memory to dengue remains poorly characterized in this region. Methods: We enumerated levels of dengue specific memory B cells (MBC), neutralizing (NT) and binding antibodies in healthy adults (n = 70) from New Delhi. Results: NT-antibodies, binding antibodies and MBC were detectable in 86%, 86.56% and 81.63% of the subjects respectively. Among the neutralizing positive subjects, 58%, 27%, 5% and 10% neutralized all four, any three, any two and any one dengue serotypes respectively. The presence of the neutralizing antibodies was associated with the presence of the MBC and binding antibodies. However, a massive interindividual variation was observed in the levels of the neutralizing antibodies (range, <1:50–1:30,264), binding antibodies (range, 1:3,000–1:134,000,) as well as the MBC (range = 0.006%–5.05%). Conclusion: These results indicate that a vast majority of the adults are immune to multiple dengue serotypes and show massive interindividual variation in neutralizing/binding antibodies and MBCs – emphasizing the importance of monitoring multiple parameters of immune memory in order to properly plan, evaluate and interpret dengue vaccines.

AB - Background: The Indian population is facing highest dengue burden worldwide supporting an urgent need for vaccines. For vaccine introduction, evaluation and interpretation it is important to gain a critical understanding of immune memory induced by natural exposure. However, immune memory to dengue remains poorly characterized in this region. Methods: We enumerated levels of dengue specific memory B cells (MBC), neutralizing (NT) and binding antibodies in healthy adults (n = 70) from New Delhi. Results: NT-antibodies, binding antibodies and MBC were detectable in 86%, 86.56% and 81.63% of the subjects respectively. Among the neutralizing positive subjects, 58%, 27%, 5% and 10% neutralized all four, any three, any two and any one dengue serotypes respectively. The presence of the neutralizing antibodies was associated with the presence of the MBC and binding antibodies. However, a massive interindividual variation was observed in the levels of the neutralizing antibodies (range, <1:50–1:30,264), binding antibodies (range, 1:3,000–1:134,000,) as well as the MBC (range = 0.006%–5.05%). Conclusion: These results indicate that a vast majority of the adults are immune to multiple dengue serotypes and show massive interindividual variation in neutralizing/binding antibodies and MBCs – emphasizing the importance of monitoring multiple parameters of immune memory in order to properly plan, evaluate and interpret dengue vaccines.

KW - Dengue

KW - India

KW - Memory B-cell

KW - Neutralizing antibody

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