Analysis of chromosome segregation in Saccharomyces cerevisiae

James H. Shero, Michael Koval, Forrest Spencer, Robert E. Palmer, Philip Hieter, Douglas Koshland

Research output: Contribution to journalArticle

Abstract

The yeast Saccharomyces cerevisiae is an excellent organism for the study of mitotic and meiotic chromosome segregation because it is possible to isolate mutations that effect the fidelity of this process. This chapter presents two methods for analyzing chromosome segregation in these mutants. In the first method, digital imaging microscopy is used on individual live cells to analyze the segregation of fluorescently labeled chromosomes. In this method, changes in nuclear DNA staining pattern are observed using a microscope equipped with epifluorescence optics suitable for DAPI-stained sampies. A low-light level imaging device, such as a silicon-intensified tube camera or a multichannel plate intensifier, is coupled to the microscope and used to obtain video images. In the second method, the construction of artificial chromosomes is described that have been specifically designed for analysis of the fidelity of chromosome segregation in mitosis and meiosis.

Original languageEnglish (US)
Pages (from-to)749-773
Number of pages25
JournalMethods in Enzymology
Volume194
Issue numberC
DOIs
StatePublished - Jan 1 1991

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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    Shero, J. H., Koval, M., Spencer, F., Palmer, R. E., Hieter, P., & Koshland, D. (1991). Analysis of chromosome segregation in Saccharomyces cerevisiae. Methods in Enzymology, 194(C), 749-773. https://doi.org/10.1016/0076-6879(91)94057-J