Analysis of chemotaxis in dictyostelium

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Dictyostelium discoideum is an excellent model organism for the study of directed cell migration, since Dictyostelium cells show robust chemotactic responses to the chemoattractant cAMP. Many powerful experimental tools are applicable, including forward and reverse genetics, biochemistry, microscopy, and proteomics. Recent studies have demonstrated that many components involved in chemotaxis are functionally conserved between human neutrophils and Dictyostelium amoebae. In this chapter, we describe how to define the functions of proteins that mediate and regulate cell motility, cell polarity, and directional sensing during chemotaxis in Dictyostelium.

Original languageEnglish (US)
Title of host publicationIntegrin and Cell Adhesion Molecules
Subtitle of host publicationMethods and Protocols
EditorsMotomu Shimaoka, Motomu Shimaoka
Pages451-468
Number of pages18
DOIs
StatePublished - Oct 24 2011

Publication series

NameMethods in Molecular Biology
Volume757
ISSN (Print)1064-3745

Keywords

  • Cell migration
  • Chemotaxis
  • Dictyostelium
  • Intracellular signaling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Cai, H., Huang, C. H., Devreotes, P. N., & Iijima, M. (2011). Analysis of chemotaxis in dictyostelium. In M. Shimaoka, & M. Shimaoka (Eds.), Integrin and Cell Adhesion Molecules: Methods and Protocols (pp. 451-468). (Methods in Molecular Biology; Vol. 757). https://doi.org/10.1007/978-1-61779-166-6_26