Abstract
There are a number of reasons to use Drosophila as a model system to study cell migration. First and foremost is the availability of an arsenal of powerful genetic techniques that can be deployed, permitting the study of cell migration in vivo, in the context of the entire organism. This is especially important for the study of a complex behavior that can be dramatically affected by small changes in environmental conditions. Several different types of cell migrations occur during Drosophila development. In this chapter, we focus on cell migrations that have been subjected to the most intense scrutiny. We describe each of the cell types and their trajectories and provide information regarding markers that are useful for the study of each cell type and mutations that affect their migrations. In addition, we provide protocols for staining embryos and manipulating gene function in each of the migratory populations. Finally, we offer some advice concerning the analysis and interpretation of mutant phenotypes.
Original language | English (US) |
---|---|
Pages (from-to) | 175-202 |
Number of pages | 28 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 294 |
State | Published - 2005 |
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ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Analysis of cell migration using Drosophila as a model system. / McDonald, Jocelyn A.; Montell, Denise J.
In: Methods in molecular biology (Clifton, N.J.), Vol. 294, 2005, p. 175-202.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of cell migration using Drosophila as a model system.
AU - McDonald, Jocelyn A.
AU - Montell, Denise J.
PY - 2005
Y1 - 2005
N2 - There are a number of reasons to use Drosophila as a model system to study cell migration. First and foremost is the availability of an arsenal of powerful genetic techniques that can be deployed, permitting the study of cell migration in vivo, in the context of the entire organism. This is especially important for the study of a complex behavior that can be dramatically affected by small changes in environmental conditions. Several different types of cell migrations occur during Drosophila development. In this chapter, we focus on cell migrations that have been subjected to the most intense scrutiny. We describe each of the cell types and their trajectories and provide information regarding markers that are useful for the study of each cell type and mutations that affect their migrations. In addition, we provide protocols for staining embryos and manipulating gene function in each of the migratory populations. Finally, we offer some advice concerning the analysis and interpretation of mutant phenotypes.
AB - There are a number of reasons to use Drosophila as a model system to study cell migration. First and foremost is the availability of an arsenal of powerful genetic techniques that can be deployed, permitting the study of cell migration in vivo, in the context of the entire organism. This is especially important for the study of a complex behavior that can be dramatically affected by small changes in environmental conditions. Several different types of cell migrations occur during Drosophila development. In this chapter, we focus on cell migrations that have been subjected to the most intense scrutiny. We describe each of the cell types and their trajectories and provide information regarding markers that are useful for the study of each cell type and mutations that affect their migrations. In addition, we provide protocols for staining embryos and manipulating gene function in each of the migratory populations. Finally, we offer some advice concerning the analysis and interpretation of mutant phenotypes.
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UR - http://www.scopus.com/inward/citedby.url?scp=21644465557&partnerID=8YFLogxK
M3 - Article
C2 - 15576913
AN - SCOPUS:21644465557
VL - 294
SP - 175
EP - 202
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -