Analysis of blood spots for polyfluoroalkyl chemicals

Kayoko Kato, Amal Wanigatunga, Larry L. Needham, Antonia M. Calafat

Research output: Contribution to journalArticle

Abstract

Polyfluoroalkyl chemicals (PFCs) have been detected in humans, in the environment, and in ecosystems around the world. The potential for developmental and reproductive toxicities of some PFCs is of concern especially to children's health. In the United States, a sample of a baby's blood, called a "dried blood spot" (DBS), is obtained from a heel stick within 48 h of a child's birth. DBS could be useful for assessing prenatal exposure to PFCs. We developed a method based on online solid phase extraction coupled with high performance liquid chromatography-isotope dilution tandem mass spectrometry for measuring four PFCs in DBS, perfluorooctane sulfonate (PFOS), perfluorohexane sulfonate, perfluorooctanoate (PFOA), and perfluorononanoate. The analytical limits of detection using one whole DBS (∼75 μL of blood) were <0.5 ng mL -1 . To validate the method, we analyzed 98 DBS collected in May 2007 in the United States. PFOS and PFOA were detected in all DBS at concentrations in the low ng mL -1 range. These data suggest that DBS may be a suitable matrix for assessing perinatal exposure to PFCs, but additional information related to sampling and specimen storage is needed to demonstrate the utility of these measures for assessing exposure.

Original languageEnglish (US)
Pages (from-to)51-55
Number of pages5
JournalAnalytica Chimica Acta
Volume656
Issue number1-2
DOIs
StatePublished - Dec 10 2009
Externally publishedYes

Fingerprint

Blood
blood
perfluorooctanoic acid
sulfonate
analysis
chemical
child health
Heel
Solid Phase Extraction
High performance liquid chromatography
Tandem Mass Spectrometry
Isotopes
Ecosystems
Dilution
Mass spectrometry
Ecosystem
Toxicity
Limit of Detection
liquid chromatography
dilution

Keywords

  • Dried blood spot
  • Perinatal exposure
  • Polyfluoroalkyl chemicals

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Analysis of blood spots for polyfluoroalkyl chemicals. / Kato, Kayoko; Wanigatunga, Amal; Needham, Larry L.; Calafat, Antonia M.

In: Analytica Chimica Acta, Vol. 656, No. 1-2, 10.12.2009, p. 51-55.

Research output: Contribution to journalArticle

Kato, Kayoko ; Wanigatunga, Amal ; Needham, Larry L. ; Calafat, Antonia M. / Analysis of blood spots for polyfluoroalkyl chemicals. In: Analytica Chimica Acta. 2009 ; Vol. 656, No. 1-2. pp. 51-55.
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