TY - JOUR
T1 - Analysis of Aichi virus and Saffold virus association with pediatric acute gastroenteritis
AU - Li, Li li
AU - Liu, Na
AU - Yu, Jei mei
AU - Ao, Yuan yun
AU - Li, Shan
AU - Stine, O. Colin
AU - Duan, Zhao jun
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Background Aichi virus (AiV) and Saffold virus (SAFV) have been reported in children with acute gastroenteritis and respiratory disease worldwide; however, their causative role in acute gastroenteritis remains ambiguous. Objectives To assess the clinical association of AiV and SAFV with acute gastroenteritis in the pediatric population. Study design A case-control study involving 461 paired stool samples from pediatric cases with diarrhea and healthy controls was conducted in China. Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) was used to screen AiV and SAFV. Results In the 461 paired samples, AiV and SAFV were more prevalent among asymptomatic children than children with acute gastroenteritis (0.87% vs. 0.43% and 2.8% vs. 1.5%, respectively), with no significant differences between groups (p = 0.142 and p = 0.478, respectively). Cox regression model analysis revealed no correlation between AiV (odds ratio, OR = 2.24; 95% confidence interval, CI, 0.76–6.54) or SAFV infection (OR = 1.36; 95% CI, 0.86–2.15) and diarrhea. High viral loads were found in both AiV- and SAFV-positive groups, with no significant difference in viral load between the groups (p = 0.507 and p = 0.677, respectively). No other known enteric pathogens were found in the AiV-positive samples but common in SAFV-positive cases. Phylogenetic analysis revealed that all 6 AiV subjects clustered with genotype B. All 7 SAFV-positive cases and 8 of 13 SAFV-positive controls were genotyped successfully; the genotypes identified included SAFV-1, SAFV-2 SAFV-3, and SAFV-6. Conclusion Our study revealed no association of these viruses in acute gastroenteritis in children. These viruses may have the ability to replicate in humans; however, the infections are usually asymptomatic.
AB - Background Aichi virus (AiV) and Saffold virus (SAFV) have been reported in children with acute gastroenteritis and respiratory disease worldwide; however, their causative role in acute gastroenteritis remains ambiguous. Objectives To assess the clinical association of AiV and SAFV with acute gastroenteritis in the pediatric population. Study design A case-control study involving 461 paired stool samples from pediatric cases with diarrhea and healthy controls was conducted in China. Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) was used to screen AiV and SAFV. Results In the 461 paired samples, AiV and SAFV were more prevalent among asymptomatic children than children with acute gastroenteritis (0.87% vs. 0.43% and 2.8% vs. 1.5%, respectively), with no significant differences between groups (p = 0.142 and p = 0.478, respectively). Cox regression model analysis revealed no correlation between AiV (odds ratio, OR = 2.24; 95% confidence interval, CI, 0.76–6.54) or SAFV infection (OR = 1.36; 95% CI, 0.86–2.15) and diarrhea. High viral loads were found in both AiV- and SAFV-positive groups, with no significant difference in viral load between the groups (p = 0.507 and p = 0.677, respectively). No other known enteric pathogens were found in the AiV-positive samples but common in SAFV-positive cases. Phylogenetic analysis revealed that all 6 AiV subjects clustered with genotype B. All 7 SAFV-positive cases and 8 of 13 SAFV-positive controls were genotyped successfully; the genotypes identified included SAFV-1, SAFV-2 SAFV-3, and SAFV-6. Conclusion Our study revealed no association of these viruses in acute gastroenteritis in children. These viruses may have the ability to replicate in humans; however, the infections are usually asymptomatic.
KW - Aichi virus
KW - Case-control
KW - Pathogenicity
KW - Real-time PCR
KW - Saffold virus
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U2 - 10.1016/j.jcv.2016.12.003
DO - 10.1016/j.jcv.2016.12.003
M3 - Article
C2 - 27992789
AN - SCOPUS:85006487060
SN - 1386-6532
VL - 87
SP - 37
EP - 42
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
ER -