Analyses of SUM1-1-mediated long-range repression

Lourdes Valenzuela, Sunil Gangadharan, Rohinton T. Kamakaka

Research output: Contribution to journalArticle

Abstract

In Saccharomyces cerevisiae, local repression is promoter specific and localized to a small region on the DNA, while silencing is promoter nonspecific, encompasses large domains of chromatin, and is stably inherited for multiple generations. Sum1p is a local repressor protein that mediates repression of meiosisspecific genes in mitotic cells while the Sir proteins are long-range repressors that stably silence genes at HML, HMR, and telomeres. The SUM1-1 mutation is a dominant neomorphic mutation that enables the mutant protein to be recruited to the HMR locus and repress genes, even in the absence of the Sir proteins. In this study we show that the mutation in Sum1-1p enabled it to spread, and the native HMR barrier blocked it from spreading. Thus, like the Sir proteins, Sum1-1p was a long-range repressor, but unlike the Sir proteins, Sum1-1p-mediated repression was more promoter specific, repressing certain genes better than others. Furthermore, repression mediated by Sum1-1p was not stably maintained or inherited and we therefore propose that Sum1-1p-mediated long-range repression is related but distinct from silencing.

Original languageEnglish (US)
Pages (from-to)99-112
Number of pages14
JournalGenetics
Volume172
Issue number1
DOIs
StatePublished - Jan 2006
Externally publishedYes

Fingerprint

Mutation
Genes
Proteins
Repressor Proteins
Telomere
Mutant Proteins
Chromatin
Saccharomyces cerevisiae
DNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Valenzuela, L., Gangadharan, S., & Kamakaka, R. T. (2006). Analyses of SUM1-1-mediated long-range repression. Genetics, 172(1), 99-112. https://doi.org/10.1534/genetics.105.050427

Analyses of SUM1-1-mediated long-range repression. / Valenzuela, Lourdes; Gangadharan, Sunil; Kamakaka, Rohinton T.

In: Genetics, Vol. 172, No. 1, 01.2006, p. 99-112.

Research output: Contribution to journalArticle

Valenzuela, L, Gangadharan, S & Kamakaka, RT 2006, 'Analyses of SUM1-1-mediated long-range repression', Genetics, vol. 172, no. 1, pp. 99-112. https://doi.org/10.1534/genetics.105.050427
Valenzuela L, Gangadharan S, Kamakaka RT. Analyses of SUM1-1-mediated long-range repression. Genetics. 2006 Jan;172(1):99-112. https://doi.org/10.1534/genetics.105.050427
Valenzuela, Lourdes ; Gangadharan, Sunil ; Kamakaka, Rohinton T. / Analyses of SUM1-1-mediated long-range repression. In: Genetics. 2006 ; Vol. 172, No. 1. pp. 99-112.
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