Analyses of promoter hypermethylation for RUNX3 and other tumor suppressor genes in nasopharyngeal carcinoma

Sing Huang Tan, Hiroshi Ida, Boon Cher Goh, Wenson Hsieh, Marie Loh, Yoshiaki Ito

Research output: Contribution to journalArticle

Abstract

Background: Aberrant methylation of cytosine in promoter CpG islands is a recognized contributory process to carcinogenesis. This study explores the methylation profile of RUNX3 in combination with p16, RASSF1A, CDH1 and hMLH1 in nasopharyngeal carcinoma (NPC) patients. Materials and Methods: Genomic DNA was extracted from 19 fresh frozen NPC biopsies, which were then subjected to bisulfite conversion and methylation-specific PCR for analysis of promoter hypermethylation for the five respective genes. Three cell lines, SNU1, RKO and LS174T, were used as controls. Results: The incidences of promoter methylation were as follows: RUNX3 0/19 (0%), p16 6/19 (32%), RASSF1A 13/19 (68%), CDH1 9/19 (47%) and hMLH1 4/19 (21%). Ninety-five percent of the tumor specimens displayed aberrant methylation in at least one of these genes. No significant correlation between methylation status of these genes and clinical parameters was found. Conclusion: Methylation of multiple genes is involved in critical pathways for cancer development in NPC. Promoter hypermethylation for RUNX3 was, however, not present.

Original languageEnglish (US)
Pages (from-to)4287-4292
Number of pages6
JournalAnticancer Research
Volume26
Issue number6 B
StatePublished - Nov 1 2006

Keywords

  • Hypermethylation
  • Nasopharyngeal cancer
  • RUNX3

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Analyses of promoter hypermethylation for RUNX3 and other tumor suppressor genes in nasopharyngeal carcinoma'. Together they form a unique fingerprint.

  • Cite this

    Tan, S. H., Ida, H., Goh, B. C., Hsieh, W., Loh, M., & Ito, Y. (2006). Analyses of promoter hypermethylation for RUNX3 and other tumor suppressor genes in nasopharyngeal carcinoma. Anticancer Research, 26(6 B), 4287-4292.