An SNF2 factor involved in mammalian development and cellular proliferation

Eric H. Raabe, Luby Abdurrahman, Greg Behbehani, Robert Arceci

Research output: Contribution to journalArticlepeer-review

Abstract

Members of the SNF2 (Sucrose Non-Fermenter) family of chromatin-remodeling proteins function in processes ranging from DNA repair to transcription to methylation. Using differential display, we recently identified a novel member of the SNF2 family that is highly expressed at the mRNA level in proliferating cells and is down-regulated during apoptosis. We have named this gene PASG (Proliferation-Associated SNF2-1ike Gene). Northern blot analysis of adult mouse tissues shows PASG to be highly expressed in proliferating organs such as thymus, bone marrow, and testis and absent from nonproliferative tissues such as brain and heart. In situ hybridization analysis of mouse embryos shows that PASG is differentially expressed during development, with highest expression in developing face, limbs, skeletal muscle, heart, and tail. In vitro, PASG expression correlates with a shift from a quiescent to a proliferative state. Mice null for PASG (also known as LSH or Hells) are reported to die perinatally, although the mechanism for lethality is unclear (Geiman and Muegge, 2000). To test the hypothesis that PASG functions in cell proliferation, we compared 5-bromodeoxyuridine (BrdU) incorporation in C33A cells transiently transfected with PASG versus empty vector and found that PASG transfected cells showed a significant decrease in the amount of BrdU incorporation. These findings suggest that PASG plays a role in cell proliferation and may function in the development of multiple cell lineages during murine embryogenesis.

Original languageEnglish (US)
Pages (from-to)92-105
Number of pages14
JournalDevelopmental Dynamics
Volume221
Issue number1
DOIs
StatePublished - May 9 2001
Externally publishedYes

Keywords

  • ATPase
  • DDM1
  • Helicase
  • Hells
  • LSH
  • Limb
  • Methylation
  • SMARCA6

ASJC Scopus subject areas

  • Developmental Biology

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