An RNA-binding protein Blanks plays important roles in defining small RNA and mRNA profiles in Drosophila testes

Susan E. Liao, Yiwei Ai, Ryuya Fukunaga

Research output: Contribution to journalArticlepeer-review

Abstract

Drosophila Blanks is a testes-specific RNA-binding protein required for post-meiotic spermiogenesis. However, Blanks's role in regulating RNA populations in the testes remains unknown. We performed small RNA and mRNA high-throughput sequencing in blanks mutant testes and controls. We identified two miRNAs, one siRNA, and hundreds of mRNAs that are significantly upregulated or downregulated in blanks mutant testes. Pathway analysis revealed that differentially expressed mRNAs are involved in catabolic and metabolic processes, anion and cation transport, mating, and reproductive behavior. Our results reveal that Blanks plays important roles in defining testicular small RNA and mRNA profiles.

Original languageEnglish (US)
Article numbere00706
JournalHeliyon
Volume4
Issue number7
DOIs
StatePublished - Jul 2018

Keywords

  • Genetics
  • Molecular biology

ASJC Scopus subject areas

  • General

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